Automated acoustic matrix deposition for MALDI sample preparation
Automated acoustic matrix deposition for MALDI sample preparation
复制标题
DOI：
10.1021/ac051534r
复制
发表时间：
2006-02-01
影响因子：
7.4
通讯作者：
Caprioli, RM
中科院分区：
文献类型：
--
作者：
Aerni, HR;Cornett, DS;Caprioli, RM
提出了用于MALDI成像质谱（IMS）和分析的新型高通量样品制备策略。开发了一种声学试剂多点定位器，以提供用于将基质沉积到样品表面（例如，组织切片）上的改进的再现性。讨论了声学液滴喷射器的独特设计及其对基体溶液沉积的优化。由于它不包含用于流体喷射的毛细管或喷嘴，因此避免了这些孔口堵塞的问题。自动基质沉积提供了对影响蛋白质提取和基质结晶的条件的更好控制，具有将基质精确存款到小表面特征上的能力。对于组织切片，获得了直径为180-200 μ m的基质斑点，并描述了用于生成质谱仪可读的坐标文件以允许自动化轮廓采集的程序。发现质谱质量和再现性优于手动移液器点样获得的质量和再现性。该仪器还可以以密集阵列图案沉积存款基质斑点，使得在质谱仪中分析之后，可以构建二维离子图像。给出了来自小鼠大脑的示例离子图像。
Novel high-throughput sample preparation strategies for MALDI imaging mass spectrometry (IMS) and profiling are presented. An acoustic reagent multispotter was developed to provide improved reproducibility for depositing matrix onto a sample surface, for example, such as a tissue section. The unique design of the acoustic droplet ejector and its optimization for depositing matrix solution are discussed. Since it does not contain a capillary or nozzle for fluid ejection, issues with clogging of these orifices are avoided. Automated matrix deposition provides better control of conditions affecting protein extraction and matrix crystallization with the ability to deposit matrix accurately onto small surface features. For tissue sections, matrix spots of 180-200 mu m in diameter were obtained and a procedure is described for generating coordinate files readable by a mass spectrometer to permit automated profile acquisition. Mass spectral quality and reproducibility was found to be better than that obtained with manual pipet spotting. The instrument can also deposit matrix spots in a dense array pattern so that, after analysis in a mass spectrometer, two-dimensional ion images may be constructed. Example ion images from a mouse brain are presented.