Identification and molecular characterization of 18 paramyxoviruses isolated from snakes
Identification and molecular characterization of 18 paramyxoviruses isolated from snakes
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DOI：
10.1016/s0168-1702(01)00353-7
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发表时间：
2001-11-28
期刊：
影响因子：
5
通讯作者：
Blahak, S
中科院分区：
文献类型：
--
作者：
Franke, J;Essbauer, S;Blahak, S
经典型细胞病变(CPE)和电子显微镜鉴定，18种不同蛇种(蛇科、蛇科和大蛇科)的病毒病原体具有呼吸道症状和神经元性疾病。和血凝抑制。通过部分L和F基因特异的逆转录聚合酶链式反应和测序、核苷酸和氨基酸序列比对以及系统发育分析(PHYLIP)对病毒进行了详细的分子特征分析。18株病毒的L基因部分序列(566nt)RT-PCR均为成功，16例均能扩增出F基因部分序列(918nt)。F-和L序列与费尔德兰斯病毒的核苷酸同源性为79%-88%，氨基酸同源性为94%-99%。对蛇毒副粘病毒的系统发育分析表明，L基因序列有3个簇，F基因序列有3个簇，表明无种属特异性。它具有相同的七肽重复序列A的保守基序，并预测了一个Furin裂解位点。这种一致性将蛇病毒群与副粘病毒亚科的其他类型物种区分开来。为了进一步分类，我们将副粘病毒和副粘病毒科成员的序列进行了比对，如仙台病毒(呼吸综合征病毒属)、腮腺炎病毒(风疹病毒属)、麻疹病毒(麻疹病毒属)、人类呼吸道合胞病毒(肺炎病毒属)(Van Regenmortel和10个合著者，2000)和Hendra病毒。它们最近被认为是亨尼帕病毒属的模式种(Wang等人，2000年)。与仙台病毒L基因部分序列同源性最高，核苷酸同源性为56%，氨基酸同源性为61%。FDLV和仙台病毒在L-和F-蛋白的系统发育分析中相对于副粘病毒模式种和亨德拉病毒表现出最密切的亲缘关系。关于生物学特性，抗原距离。特别是现有序列的低同源性，我们提出了副粘病毒科内爬行动物副粘病毒的一个新属。(C)2001 Elsevier Science B.V.保留所有权利。
Viral agents from 18 different snake species (families Colubridae., Viperidae, and Crotalidae) showing respiratory symptoms and neuronal disease were identified as paramyxoviruses by typical cytopathogenic effect (CPE), electron microscopy. and hemagglutination inhibition. Detailed molecular characterization of the viruses was performed by partial L- and F-gene-specific reverse transcription polymerase chain reaction (RT-PCR) and sequencing, nucleotide and amino acid sequence alignment, and phylogenetic analysis (PHYLIP). RT-PCR of the partial L-gene (566 nt) was successful for all 18 viruses, amplicons of the partial F-gene (918 nt) could be obtained in 16 cases. F- and L-sequence alignment revealed similarities to Fer de Lance virus (FDLV) ranging from 79 to 88% on a nucleotide basis, and 94 to 99% on an amino acid basis. Phylogenetic analysis of the ophidian paramyxoviruses resulted in three clusters for the L-gene sequence and corresponding clusters for the F-gene sequence, indicating no species specificity, We analyzed the F-protein of the snake paramyxoviruses. which proved to have an identical conserved motif of heptad repeat A and predicted a furin cleavage site. This uniformity distinguishes the snake virus group from the other type species of the subfamily Paramyxovirinae. For further classification., we aligned the sequences of the ophidian paramyxoviruses and members of the Paramyxoviridae, such as Sendai virus (genus Respirovirus), mumps virus (genus Rubulavirus), measles virus (genus Morbillivirus), human respiratory syncytial virus (genus Pneumovirus) (Van Regenmortel and 10 co-authors, 2000) and Hendra virus. which have recently been suggested as type species of the genus Henipavirus (Wang et al., 2000). Maximum sequence similarity was found to the partial L-gene of Sendai virus, with 56% nucleotide and 61% amino acid identity. The FDLV and Sendai virus cluster in the phylogenetic analysis Of L- and F-protein regarding the Paramyxovirus type species and Hendra virus and show the closest relationship. Regarding the biological properties, the antigenic distance. and particularly the low homology of available sequences, we propose a new genus for the reptilian paramyxoviruses within the Paramyxoviridae. (C) 2001 Elsevier Science B.V. All rights reserved.