Engineered Biosynthesis of 5/5/6 Type Polycyclic Tetramate Macrolactams in an Ikarugamycin (5/6/5 Type)-Producing Chassis
Engineered Biosynthesis of 5/5/6 Type Polycyclic Tetramate Macrolactams in an Ikarugamycin (5/6/5 Type)-Producing Chassis
复制标题
在斑鸠霉素（5/6/5 型）生产底盘中进行 5/5/6 型多环四酸酯大环内酰胺的工程生物合成
DOI：
10.1021/acs.orglett.9b04672
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发表时间：
2020-03-06
期刊：
影响因子：
5.2
通讯作者：
Zhang, Changsheng
中科院分区：
文献类型：
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作者：
Jin, Hongbo;Zhang, Wenjun;Zhang, Changsheng
通过引入一组来自griseus链霉菌（Streptornyces griseus）的5/5/6型PoTeM生物合成基因，在工程ikarugamycin（5/6/5型）生产装置中实现了5/5/6型多环四聚内酰胺（polycyclic tetrate macrolactams, PoTeM）的组合生物合成。该研究支持了PoTeMs具有由杂化聚酮合成酶/非核糖体肽合成酶产生的共同多烯四聚酸前体的观点。pactamide G(7)的x射线晶体结构为解析5/5/6型potem的绝对构型提供了范例。
Combinatorial biosynthesis of 5/5/6 type polycyclic tetramate macrolactams (PoTeMs) was achieved in an engineered ikarugamycin (5/6/5 type) producer by introducing a set of 5/5/6 type PoTeM biosynthetic genes from Streptornyces griseus. This study supports the idea that PoTeMs share a common polyene tetramate precursor generated by the hybrid polyketide synthase/nonribosomal peptide synthetase enzymes. The X-ray crystal structure of pactamide G (7) sets an example for resolving the absolute configuration of 5/5/6 type PoTeMs.