PCR as a diagnostic tool for brucellosis
PCR as a diagnostic tool for brucellosis
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DOI：
10.1016/s0378-1135(02)00228-6
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发表时间：
2002-12-20
影响因子：
3.3
通讯作者：
Bricker, BJ
中科院分区：
文献类型：
--
作者：
Bricker, BJ
已经开发了许多基于PCR的检测方法来鉴定布鲁氏菌，以提高诊断能力。总的来说，测定库解决了诊断过程的几个方面。对于某些目的，布鲁氏菌的简单鉴定就足够了（例如，人类布鲁氏菌病或食品污染的诊断）。在这些情况下，属特异性PCR测定就足够了。属特异性测定往往是简单的，稳健的，并且在一定程度上允许环境影响。用于这些应用的主要遗传靶标是布鲁氏菌BCSP 31基因和16 S-23 S rRNA操纵子。例如，大多数政府资助的布鲁氏菌病根除计划包括规定物种特异性反应的法规。对于流行病学追溯，菌株特异性鉴定是有帮助的。通常，基于差异PCR的测定往往更复杂，因此更难以进行。已经探索了几种策略来区分布鲁氏菌物种和菌株，包括基因座特异性多重（例如基于IS 711的AMOS-PCR）、PCR-RFLP（例如omp 2基因座）、任意引物PCR和ERIC-PCR，仅举几例。本文综述了布鲁氏菌分子诊断的一些主要进展，包括用于直接分析各种临床样品的程序的开发。虽然迄今取得的进展令人印象深刻，但仍有改进的余地。(C)2002 Elsevier Science B. V.保留所有权利。
Numerous PCR-based assays have been developed for the identification of Brucella to improve diagnostic capabilities. Collectively, the repertoire of assays addresses several aspects of the diagnostic process. For some purposes, the simple identification of Brucella is adequate (e.g. diagnosis of human brucellosis or contamination of food products). In these cases, a genus-specific PCR assay is sufficient. Genus-specific assays tend to be simple, robust, and somewhat permissive of environmental influences. The main genetic targets utilized for these applications are the Brucella BCSP31 gene and the 16S-23S rRNA operon.Other instances require identification of the Brucella species involved. For example, most government-sponsored brucellosis eradication programs include regulations that stipulate a species-specific response. For epidemiological trace back, strain-specific identification is helpful. Typically, differential PCR-based assays tend to be more complex and consequently more difficult to perform. Several strategies have been explored to differentiate among Brucella species and strains, including locus specific multiplexing (e.g. AMOS-PCR based on IS711), PCR-RFLP (e.g. the omp2 locus), arbitrary-primed PCR, and ERIC-PCR to name a few. This paper reviews some of the major advancements in molecular diagnostics for Brucella including the development of procedures designed for the direct analysis of a variety of clinical samples. While the progress to date is impressive, there is still room for improvement. (C) 2002 Elsevier Science B.V. All rights reserved.