Post-translational processing of thyrotropin-releasing hormone precursor in rat brain: identification of 3 novel peptides derived from proTRH.
Post-translational processing of thyrotropin-releasing hormone precursor in rat brain: identification of 3 novel peptides derived from proTRH.
复制标题
大鼠脑中促甲状腺素释放激素前体的翻译后加工：从 proTRH 衍生的 3 种新型肽的鉴定。
DOI：
10.1016/0006-8993(88)90342-3
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发表时间：
1988
期刊：
影响因子：
2.9
通讯作者：
Jackson,IM
中科院分区：
文献类型：
--
作者：
Wu,P;Jackson,IM
大鼠下丘脑促甲状腺激素释放激素原序列除5个TRH前体序列外，还包括前导序列、尾部序列和4个中间序列，中间由两对碱性氨基酸序列隔开。我们已经建立了与这些隐蔽的proTRH序列的部分对应的合成肽的放射免疫分析方法，并使用这些分析方法来鉴定和部分表征大鼠脑提取液中不同于TRH的proTRH多肽。这些多肽中的两个在大小上与一个插入序列和ProTRH的CAR☐y-末端序列密切对应。另外三个多肽对应于完整的氨基末端前导序列，以及两个由内部成对的碱性氨基酸序列的前导序列进一步切割而形成的多肽。
The sequence of rat hypothalamic pro-thyrotropin releasing hormone, deduced by sequencing of cDNA, in addition to 5 TRH progenitor genitor sequences contains leader, trailer and 4 intervening sequences separated by paired basic amino acid sequences. We have developed radioimmunoassays to synthetic peptides corresponding to portions of these cryptic proTRH sequences and have used these assays to identify and partially characterize proTRH peptides, distinct from TRH, in extracts of rat brain. Two of these peptides correspond closely in size to one intervening sequence and the car☐y-terminal sequence of proTRH. Three other peptides correspond to the intact amino-terminal leader sequence and two peptides formed by a further cleavage of the leader sequence at an internal paired basic amino acid sequence.