Thiol-reactive luminescent chelates of terbium and europium
Thiol-reactive luminescent chelates of terbium and europium
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DOI：
10.1021/bc980113w
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发表时间：
1999-03-01
影响因子：
4.7
通讯作者：
Selvin, PR
中科院分区：
文献类型：
--
作者：
Chen, JY;Selvin, PR
合成了具有硫醇活性的稀土络合物，当它们与Tb和/或Eu结合时会发光。这些络合物由二亚乙三胺五乙酸酯(DTPA)螯合物组成，它通过一个酰胺键共价连接到生色团Carstril 124上，并通过第二个酰胺键连接到马来酰亚胺、溴乙酰胺或吡啶二硫杂环上。介绍了与DNA激活蛋白NTRC、肌球蛋白上的不同部位或DNA结合的复合体的特定部位的附着和表征。当在螯合物和硫醇反应部分之间使用酰肼间隔物时，这些化合物与Tb的连接位点的数量惊人地多，尽管当使用Eu时，这种额外的连接可能会导致猝灭。合成是一个简单的两步或三步反应，提纯很简单。这些化合物应该是有用的非同位素替代物，在成像中作为长寿命的探针，在发光共振能量转移中作为供体。它们是一大类可以通过现成的异双官能团或同双官能团连接物进行偶联的螯合物的例子。
Thiol-reactive lanthanide complexes have been synthesized that are luminescent when bound to terbium and/or europium. The complexes consist of a diethylenetriaminepentaacetate (DTPA) chelate covalently joined through one amide bond to a chromophore, carbostyril 124, and via a second amide bond to a maleimide, bromoacetamide, or pyridyldithio moiety. Site-specific attachment and characterization of the complexes attached to DNA-activating protein NtrC, to various sites on myosin, or to DNA are presented. The compounds coordinate a surprisingly large number of ligation sites of terbium when a hydrazide spacer is used between the chelate and thiol-reactive moiety, although this extra ligation can cause quenching when europium is used. Synthesis is a simple two- or three-step reaction, and purification is straightforward. The compounds should be useful as nonisotopic replacements, as long-lifetime probes in imaging, and as donors in luminescence resonance energy transfer. They are examples of a wide class of chelates that can be made conjugatable via readily available hetero- or homo-bifunctional linkers.