Genetic and Physiological Characterization of the Intestinal Bacterial Microbiota of Bluegill (Lepomis macrochirus) with Three Different Feeding Habits
Genetic and Physiological Characterization of the Intestinal Bacterial Microbiota of Bluegill (Lepomis macrochirus) with Three Different Feeding Habits
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DOI：
10.1007/s00248-006-9018-z
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发表时间：
2006-04
影响因子：
3.6
通讯作者：
K. Uchii;K. Matsui;R. Yonekura;K. Tani;T. Kenzaka;M. Nasu;Z. Kawabata
中科院分区：
文献类型：
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作者：
K. Uchii;K. Matsui;R. Yonekura;K. Tani;T. Kenzaka;M. Nasu;Z. Kawabata
蓝鳃太阳鱼（Lepomis macrochirus）生活在日本的琵琶湖，以底栖无脊椎动物（底食型）、水生植物（草食型）和浮游动物（食草型）为食。为了评估食物对肠道细菌微生物群的影响，我们在群落水平生理特征（CLPP）和遗传结构方面对这三种类型的蓝鳃鱼的肠道微生物群进行了表征和比较。使用Biolog MicroPlates（Biolog，Inc.，海沃德，CA，USA），并且多变量方差分析揭示，在三种类型的蓝鳃鱼的任何对之间，肠道微生物群的CLPP显著不同。通过聚合酶链反应（PCR）扩增的16S rDNA片段的温度梯度凝胶电泳的遗传图谱进行了分析，和多维缩放表明存在特定的肠道细菌结构的底栖和寄生类型。这些结果表明，在自然环境中，宿主的摄食习惯可能是控制鱼类肠道菌群的一个因素。
Bluegill (Lepomis macrochirus) in Lake Biwa, Japan, feed on benthic invertebrates (benthivorous type), aquatic plants (herbivorous type), and zooplankton (planktivorous type). To evaluate the effect of food on intestinal bacterial microbiota, we characterized and compared the intestinal microbiota of these three types of bluegill in terms of community-level physiological profile (CLPP) and genetic structure. The CLPP was analyzed using Biolog MicroPlates (Biolog, Inc., Hayward, CA, USA), and multivariate analysis of variance revealed that the CLPP of intestinal microbiota differed significantly between any pairs of the three types of bluegill. The genetic profiles were analyzed by temperature gradient gel electrophoresis of polymerase chain reaction (PCR)-amplified 16S rDNA fragments, and multidimensional scaling indicated the existence of specific intestinal bacterial structures for both the benthivorous and the planktivorous types. These results suggest that the host's feeding habit can be one factor controlling the intestinal microbiota of fish in the natural environment.