Expression of pro- and anti-inflammatory cytokines and chemokines during the ovulatory cycle and effects of aging on their expression in the uterine mucosa of laying hens
Expression of pro- and anti-inflammatory cytokines and chemokines during the ovulatory cycle and effects of aging on their expression in the uterine mucosa of laying hens
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DOI：
10.1016/j.cyto.2018.09.015
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发表时间：
2018-11-01
期刊：
影响因子：
3.8
通讯作者：
Yoshimura, Y.
中科院分区：
文献类型：
--
作者：
Elhamouly, M.;Nii, T.;Yoshimura, Y.
本研究的目的是检查子宫粘膜表达的细胞因子和趋化因子是否在鸡子宫蛋壳形成过程中发挥作用。研究了排卵周期期间子宫粘膜促炎和抗炎细胞因子和趋化因子表达水平的变化（实验1）以及衰老对其表达的影响（实验2）。在实验1中，发现促炎细胞因子IL1β、IL6、TNFSF15和IFNγ以及趋化因子CX3CL1的表达在蛋壳生物矿化期间（产卵后16小时）增加，而抗炎TGFβ2表达在产卵后4小时增加。在实验2中，在老年母鸡中观察到抗炎细胞因子TGFβ2和TGFβ3以及趋化因子CXCLi2和CX3CL1的表达高于年轻母鸡。在老年母鸡的子宫组织中观察到巨噬细胞和 CD8 + T 细胞的数量显着高于年轻母鸡。此外，发现老年母鸡中与白细胞浸润相关的粘附分子的表达高于年轻母鸡。我们得出的结论是，蛋壳形成过程可能受到促炎和抗炎细胞因子和趋化因子的影响。这些分子的平衡表达在老年母鸡中可能会被破坏。
The aim of this study was to examine whether cytokines and chemokines expressed in the uterine mucosa play a role in the process of eggshell formation in the chicken uterus. Changes in the expression levels of pro- and anti-inflammatory cytokines and chemokines in the uterine mucosa during an ovulatory cycle (experiment 1) and effects of aging on their expression (experiment 2) were examined. In experiment 1, the expression of the pro inflammatory cytokines IL1 beta, IL6, TNFSF15, and IFN gamma, and a chemokine CX3CL1 was found to increase during eggshell biomineralization (16 h following oviposition), while anti-inflammatory TGF beta 2 expression was found to increase at 4 h following oviposition. In experiment 2, a higher expression of the anti-inflammatory cytokines TGF beta 2 and TGF beta 3, and chemokines CXCLi2 and CX3CL1, was observed in aged hens than in young hens. A significantly higher number of macrophages and CD8 + T cells were observed in the uterine tissue of aged hens than in young hens. Furthermore, the expression of adhesion molecules associated with leukocytic infiltration was found to be higher in aged hens than in young hens. We conclude that the eggshell formation process may be affected by the pro- and anti-inflammatory cytokines and chemokines. The balanced expressions of these molecules might be disrupted in aged hens.