The PLC1 encoded phospholipase C in the yeast Saccharomyces cerevisiae is essential for glucose-induced phosphatidylinositol turnover and activation of plasma membrane H+-ATPase
The PLC1 encoded phospholipase C in the yeast Saccharomyces cerevisiae is essential for glucose-induced phosphatidylinositol turnover and activation of plasma membrane H+-ATPase
复制标题
DOI：
10.1016/s0167-4889(98)00099-8
复制
发表时间：
1998-10-21
影响因子：
5.1
通讯作者：
Thevelein, JM
中科院分区：
文献类型：
--
作者：
Coccetti, P;Tisi, R;Thevelein, JM
将葡萄糖添加到酿酒酵母的葡萄糖剥夺细胞中会引发磷脂酰肌醇、磷脂酰肌醇磷酸和磷脂酰肌醇 4,5-二磷酸的快速周转。葡萄糖对PI周转的刺激通过P-32标记的特定比率的增加和添加葡萄糖后二酰基甘油水平的增加来测量。葡萄糖还会引起质膜 H+-ATP 酶的快速激活。我们表明，在缺乏PLC1编码的磷脂酶C的突变体中，这两个过程都大大减少。化合物 48/80 是一种已知的哺乳动物磷脂酶 C 抑制剂，可抑制这两个过程。然而，质膜 H+-ATP 酶的激活仅受到强烈抑制磷脂周转的化合物 48/80 浓度的抑制。甚至更低的浓度也会抑制生长。我们的数据表明，在酵母细胞中，葡萄糖通过 PLC1 基因产物的激活触发信号通路，该信号通路由磷脂酰肌醇周转启动并参与质膜 H+-ATP 酶的激活。 (C) 1998 Elsevier Science B.V. 保留所有权利。
Addition of glucose to glucose-deprived cells of the yeast Saccharomyces cerevisiae triggers rapid turnover of phosphatidylinositol, phosphatidylinositol-phosphate and phosphatidylinositol 4,5-bisphosphate. Glucose stimulation of PI turnover was measured both as an increase in the specific ratio of P-32-labeling and as an increase in the level of diacylglycerol after addition of glucose. Glucose also causes rapid activation of plasma membrane H+-ATPase. We show that in a mutant lacking the PLCl encoded phospholipase C, both processes were strongly reduced. Compound 48/80, a known inhibitor of mammalian phospholipase C, inhibits both processes. However, activation of the plasma membrane H+-ATPase is only inhibited by concentrations of compound 48/80 that strongly inhibit phospholipid turnover. Growth was inhibited by even lower concentrations. Our data suggest that in yeast cells, glucose triggers through activation of the PLC1 gene product a signaling pathway initiated by phosphatidylinositol turnover and involved in activation of the plasma membrane H+-ATPase. (C) 1998 Elsevier Science B.V. All rights reserved.