The mGlu5 metabotropic glutamate receptor is expressed in zones of active neurogenesis of the embryonic and postnatal brain
The mGlu5 metabotropic glutamate receptor is expressed in zones of active neurogenesis of the embryonic and postnatal brain
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DOI：
10.1016/j.devbrainres.2004.02.003
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发表时间：
2004-05-19
期刊：
影响因子：
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通讯作者：
Nicoletti, F
中科院分区：
文献类型：
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作者：
Gerevini, VDD;Caruso, A;Nicoletti, F
代谢型谷氨酸（mGlu）受体参与发育可塑性的调节。在这里，我们研究了mGlula-b，-2，-3，-4a-b，和-5a受体亚型的表达从胚胎第12天（E12）的早期和晚期出生后的生活。虽然所有的成绩单（与mGlu 4 mRNA的例外）产前检测，只有mGlu 5受体蛋白被发现在胚胎脑中的可检测量。免疫组织化学分析表明，mGlu 5受体主要表达的脑室周围的细胞在E15，而它更广泛地表达在E18。在出生后的生活中，除了其经典的表达位点，mGlu 5受体被发现在活跃的神经发生区，如小脑皮质的外部颗粒层（EGL）和脑室下区。在这些区域中，通过BrdU染色检测到活跃增殖的祖细胞的存在。在BrdU（+）细胞富集区没有发现其他亚型（在我们检查的那些亚型中）表达。这些数据表明mGlu 5受体在早期脑发育和基本细胞过程如增殖和/或分化中的作用。(C)2004 Elsevier B.V保留所有权利。
Metabotropic glutamate (mGlu) receptors have been implicated in the regulation of developmental plasticity. Here, we examined the expression of mGlula-b, -2, -3, -4a-b, and -5a receptor subtypes from embryonic day 12 (E12) to the early and late postnatal life. While all transcripts (with the exception of mGlu4 mRNA) were detected prenatally, only the mGlu5 receptor protein was found in detectable amounts in the embryonic brain. Immunohistochemical analysis showed that the mGlu5 receptor was mainly expressed by cells surrounding the ventricles at E15, whereas it was more diffusely expressed at E18. In the postnatal life, besides its classical expression sites, the mGlu5 receptor was found in zones of active neurogenesis such as the external granular layer (EGL) of the cerebellar cortex and the subventricular zone. In these regions, the presence of actively proliferating progenitor cells was detected by BrdU staining. No other subtype (among those we have examined) was found to be expressed in regions enriched of BrdU(+) cells. These data suggest a role for mGlu5 receptors ill the early brain development and in basic cellular processes such as proliferation and/or differentiation. (C) 2004 Elsevier B.V All rights reserved.