Monoclonal antibodies to discrete regions of human Glu1-plasminogen.
Monoclonal antibodies to discrete regions of human Glu1-plasminogen.
复制标题
针对人 Glu1-纤溶酶原离散区域的单克隆抗体。
DOI：
10.1021/bi00266a025
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发表时间：
1982
期刊：
影响因子：
2.9
通讯作者：
Castellino,FJ
中科院分区：
文献类型：
--
作者：
Ploplis,VA;Cummings,HS;Castellino,FJ
维多利亚A. Ploplis，* Helen S. Cummings和弗朗西斯J. Castellino* 摘要：从由高度免疫小鼠脾淋巴细胞与小鼠NS-1骨髓瘤细胞融合而成的稳定杂交细胞系中制备了人谷氨酰胺纤溶酶原不同结构域的单克隆抗体。一个细胞系10-F1产生了反应同样良好的单克隆抗体（括号内为KD值）与谷氨酰胺-纤溶酶原[（1.9±0.6）× 10~ 8 M]，Lys 77-纤溶酶原[（1.8±0.4）× 10 ~（-8）M]和Val 354-纤溶酶原[（1.0±0.4）× 10 ~（-8）M]，该抗体与纤溶酶原kringle 4 [（2.1±0.6）× 10~（-8）M]及其它所有含kringle 4的纤溶酶原片段反应，Kringles 1-4 [（2.0±0.6）× 10 ~（-8）M]和Lys 77-纤溶酶重链[（2.7±0.6）× 10~（-8）M]。这些数据表明，抗体10-F1对Kringle 4结构域上的表位具有特异性，并且该表位在所有上述含有Kringle 4的片段中是保守的。该抗体为IgG 1/类，并显示一种等电点形式，pI= 6.6±0.1。另一细胞系10-V1产生的单克隆抗体与Glupplasminogen反应良好[（0.93±0.08）× 10 ~（-8）M]，
Victoria A. Ploplis,* Helen S. Cummings, and Francis J. Castellino* abstract: Monoclonal antibodiesto various domains of hu-man Glurplasminogen have been prepared from stable hybrid cell lines consisting of hyperimmune mouse spleen lymphocytes fused to mouse NS-1 myeloma cells. One cell line, 10-Fl, produced a monoclonal antibody which reacted equally well (KD values in parentheses) with Glut-plasminogen [(1.9±0.6) X 10~ 8 M], Lys77-plasminogen [(1.8±0.4) X 10'8 M], andVal354-plasminogen [(1.0±0.4) X 10" 8 M], In addition, this antibody reacted with isolated plasminogen kringle 4 [(2.1±0.6) X 10~ 8 M] and all other isolated plasminogen fragments containing kringle 4, viz., kringles 1-4 [(2.0±0.6) X 10 “8 M] and the Lys77-plasmin heavy chain [(2.7±0.6) X 10~ 8 M], The antibody did not react with Val442-plasminogen or with kringles 1-3. These data show that the antibody 10-Fl was specific for an epitope on the kringle 4 domain and that this epitope is conserved in all of theabove fragmentscontaining kringle 4. This antibody was of the IgGi/class andexhibited one isoelectric form of pi= 6.6±0.1. Another cell line, 10-Vl, produced a monoclonal antibody which reacted equally well with Glupplasminogen [(0.93±0.08) X 10'8 M],