OVUM TRANSPORT IN THE RAT OVIDUCTAL AMPULLA IN THE ABSENCE OF MUSCLE CONTRACTILITY
OVUM TRANSPORT IN THE RAT OVIDUCTAL AMPULLA IN THE ABSENCE OF MUSCLE CONTRACTILITY
复制标题
DOI：
10.1095/biolreprod40.6.1131
复制
发表时间：
1989-06-01
影响因子：
3.6
通讯作者：
SZAL, SE
中科院分区：
文献类型：
--
作者：
HALBERT, SA;BECKER, DR;SZAL, SE
哺乳动物输卵管中的卵子运输涉及两个主要效应器：纤毛运动和肌肉收缩。为了研究纤毛对大鼠卵子运输的相对贡献，我们用异丙肾上腺素（一种β-肾上腺素能激动剂）阻断平滑肌活性，并原位测量代孕卵子的运输率。异丙肾上腺素给药前，头壶腹和尾壶腹的转运速率分别为0.04 mm/s和0.03 mm/s;异丙肾上腺素给药后，转运速率保持不变。为了确定异丙肾上腺素是否影响纤毛活动，我们在有效的异丙肾上腺素剂量下用激光散射光谱法测量纤毛搏动频率。异丙肾上腺素未引起纤毛搏动频率的显著变化。我们的研究结果表明，在大鼠输卵管壶腹，纤毛运动是能够运输卵子的肌肉收缩的情况下。
Ovum transport in mammalian oviducts involves two main effectors: ciliary motility and muscle contractility. To study the relative contribution of cilia to ovum transport in the rat, we blocked smooth muscle activity with isoproterenol, a beta-adrenergic agonist, and measured transport rates of surrogate ova in situ. Transport rates before isoproterenol administration were 0.04 mm/s in the cephalic ampulla and 0.03 mm/s in the caudal ampulla; rates were unchanged after administration of isproterenol. To determine if isoproterenol affected ciliary activity, we measured ciliary beat frequency with laser-scattering spectroscopy over the effective isoproterenol dosage. Isoproterenol did not cause a significant change in ciliary beat frequency. Our results show that in the rat oviductal ampulla, ciliary motion is capable of transporting ova in the absence of muscle contractility.