The reproductive tracts of two malaria vectors are populated by a core microbiome and by gender- and swarm-enriched microbial biomarkers.
The reproductive tracts of two malaria vectors are populated by a core microbiome and by gender- and swarm-enriched microbial biomarkers.
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DOI：
10.1038/srep24207
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发表时间：
2016-04-18
影响因子：
4.6
通讯作者：
Catteruccia F
中科院分区：
文献类型：
--
作者：
Segata N;Baldini F;Pompon J;Garrett WS;Truong DT;Dabiré RK;Diabaté A;Levashina EA;Catteruccia F
微生物在塑造昆虫的生理机能方面发挥着关键作用，并能影响行为、繁殖和对病原体的易感性。在撒哈拉以南非洲，两种主要的疟疾媒介：冈比亚按蚊和按蚊。 coluzzii 在不同的幼虫栖息地中繁殖，这些栖息地具有不同的微生物，可能会影响其成虫的生理机能，并可能影响疟原虫的传播。我们分析了雄性和雌性 An 的生殖微生物组。冈比亚和安.从布基纳法索的自然交配群中收集的 coluzzii 夫妇。对解剖组织的 16S rRNA 测序表明，生殖道含有复杂的微生物组，其特征是两个物种和所有生殖组织共有的一大群核心细菌。有趣的是，我们在特定组织中检测到了几种与性别相关的微生物生物标志物的显着富集，令人惊讶的是，从一个交配群捕获的雄性中也发现了类似的细菌类别，这表明这些雄性起源于同一幼虫繁殖地。最后，我们鉴定了几种内共生细菌，包括螺原体，它们具有操纵昆虫繁殖成功的能力。我们的研究对重要的人类疾病媒介的生殖微生物组进行了全面分析，并确定了一组核心细菌和内共生细菌，这些细菌可用于干扰按蚊传播疟疾寄生虫。
Microbes play key roles in shaping the physiology of insects and can influence behavior, reproduction and susceptibility to pathogens. In Sub-Saharan Africa, two major malaria vectors, Anopheles gambiae and An. coluzzii, breed in distinct larval habitats characterized by different microorganisms that might affect their adult physiology and possibly Plasmodium transmission. We analyzed the reproductive microbiomes of male and female An. gambiae and An. coluzzii couples collected from natural mating swarms in Burkina Faso. 16S rRNA sequencing on dissected tissues revealed that the reproductive tracts harbor a complex microbiome characterized by a large core group of bacteria shared by both species and all reproductive tissues. Interestingly, we detected a significant enrichment of several gender-associated microbial biomarkers in specific tissues, and surprisingly, similar classes of bacteria in males captured from one mating swarm, suggesting that these males originated from the same larval breeding site. Finally, we identified several endosymbiotic bacteria, including Spiroplasma, which have the ability to manipulate insect reproductive success. Our study provides a comprehensive analysis of the reproductive microbiome of important human disease vectors, and identifies a panel of core and endosymbiotic bacteria that can be potentially exploited to interfere with the transmission of malaria parasites by the Anopheles mosquito.