Changes in lipid metabolism by soy beta-conglycinin-derived peptides in HepG2 cells.
Changes in lipid metabolism by soy beta-conglycinin-derived peptides in HepG2 cells.
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DOI：
10.1021/jf8031793
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发表时间：
2009-01
影响因子：
6.1
通讯作者：
Y. Mochizuki;M. Maebuchi;M. Kohno;M. Hirotsuka;Hiroyuki Wadahama;T. Moriyama;T. Kawada;R. Urade
中科院分区：
文献类型：
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作者：
Y. Mochizuki;M. Maebuchi;M. Kohno;M. Hirotsuka;Hiroyuki Wadahama;T. Moriyama;T. Kawada;R. Urade
本研究用不含脂蛋白的高纯度大豆β-伴大豆球蛋白(7S)提取的短肽(7S-肽)处理人肝癌细胞株HepG2，观察其对细胞脂质代谢的影响。7S肽可抑制细胞分泌载脂蛋白B-100。7S肽还抑制(3)H-甘油和(14)C-乙酸酯与三酰甘油的结合，但不抑制磷脂酰胆碱和磷脂酰乙醇胺等主要磷脂的结合。此外，加入7S-肽后2 h，胆固醇酯的合成显著减少，而加入7S-肽后4 h胆固醇合成无明显变化，8 h后胆固醇合成显著增加。加入7S多肽后8h，SREBP-2裂解核型增加，表明细胞内胆固醇水平降低。对7S多肽处理后mRNA表达变化的分析表明，7S多肽降低了内质网中的胆固醇水平，增加了脂肪酸β氧化相关基因的mRNA，并促进了胆固醇的合成。这些结果表明，7S多肽可能改变了人肝癌细胞的脂代谢，从而减少了含有载脂蛋白B-100的脂蛋白的分泌。
In this study, HepG2 cells were treated with short peptides (7S-peptides) derived from highly purified soybean beta-conglycinin (7S), which was free from lipophilic protein, and the effect of the peptide treatment on lipid metabolism was determined. 7S-peptide treatment suppressed the secretion of apolipoprotein B-100 from HepG2 cells into the medium. The 7S-peptides also suppressed the incorporation of (3)H-glycerol and (14)C-acetate into triacylglyceride but not into major phospholipids, such as phosphatidylcholine and phosphatidylethanolamine. Additionally, the synthesis of cholesterol esters was dramatically decreased for 2 h after the addition of the 7S-peptides, whereas the synthesis of cholesterol remained unchanged by 4 h and increased by 8 h after the addition of the 7S-peptides. The cleaved nuclear form of SREBP-2 increased 8 h after the addition of the 7S peptides, suggesting a decrease in intracellular cholesterol levels. Analysis of changes in mRNA expression after 7S-peptide treatment suggested that the 7S-peptides lower the level of cholesterol in the endoplasmic reticulum, increase the mRNA of genes related to beta-oxidation of fatty acids, and increase the synthesis of cholesterol. From these results, it may be concluded that the peptides derived from 7S altered the lipid metabolism to decrease secretion of apolipoprotein B-100-containing lipoprotein from HepG2 cells.