Use of aequorin-based indicators for monitoring Ca2+ in acidic organelles
Use of aequorin-based indicators for monitoring Ca2+ in acidic organelles
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使用基于水母发光蛋白的指示剂监测酸性细胞器中的 Ca2
DOI：
10.1016/j.bbamcr.2023.119481
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Alonso M
中科院分区：
文献类型：
--
作者：
Alonso M
在过去的几年里，越来越多的证据表明酸性细胞器可以在细胞激活时积累和释放Ca2+。因此，在这些室室Ca2+动力学的可靠记录是必要的，以了解酸性细胞器的生理病理方面。基因编码Ca2+指示剂（GECIs）是监测特定位置Ca2+的有价值的工具，尽管由于大多数可用的荧光GECIs的pH敏感性，它们在酸性隔间中的使用具有挑战性。相比之下，生物发光GECIs具有多种特性（边际pH敏感性、低背景、无光毒性、无光漂白、高动态范围和可调亲和力），这使得它们有利于在酸性隔间中实现增强的信噪比。本文综述了针对酸性腔室的生物发光水藻蛋白基GECIs的应用。需要在高酸性隔间中进行更多的测量。
Over the last years, there is accumulating evidence that acidic organelles can accumulate and release Ca2+upon cell activation. Hence, reliable recording of Ca2+dynamics in these compartments is essential for understanding the physiopathological aspects of acidic organelles. Genetically encoded Ca2+indicators (GECIs) are valuable tools to monitor Ca2+in specific locations, although their use in acidic compartments is challenging due to the pH sensitivity of most available fluorescent GECIs. By contrast, bioluminescent GECIs have a combination of features (marginal pH sensitivity, low background, no phototoxicity, no photobleaching, high dynamic range and tunable affinity) that render them advantageous to achieve an enhanced signal-to-noise ratio in acidic compartments. This article reviews the use of bioluminescent aequorin-based GECIs targeted to acidic compartments. A need for more measurements in highly acidic compartments is identified.