Surface functionalization of a polymeric lipid bilayer for coupling a model biological membrane with molecules
Surface functionalization of a polymeric lipid bilayer for coupling a model biological membrane with molecules
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用于将模型生物膜与分子耦合的聚合物脂质双层的表面功能化
DOI：
10.1021/la304747e
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
H. Imaishi
中科院分区：
文献类型：
--
作者：
K. Morigaki;K. Mizutani;M. Saito;T. Okazaki;Y. Nakajima;Y. Tatsu;H. Imaishi
我们描述了一个稳定的和功能的模型生物膜基于聚合脂双分子层与化学修饰的表面。由两种含二乙炔的磷脂，1,2-二（10,12-三osadiynoyl）-sn-甘油-3-磷酸胆碱（DiynePC）和1,2-二（10,12-三osadiynoyl）-sn-甘油-3-磷酸乙醇胺（DiynePE）的混合物形成聚合脂质双分子层。DiynePC形成了稳定的双层结构，而DiynePE的乙醇胺头基使功能分子能够接枝到膜表面。DiynePC和DiynePE的共聚形成了坚固的双分子层。聚合物双分子层的功能化提供了一条通往强大的仿生表面的途径，可以与生物分子、细胞和三维（3D）微结构连接。将生物素和多肽分别接枝到聚合物双分子层上，分别与链亲和素和培养的哺乳动物细胞进行分子识别。蛋白质和细胞在聚合物双分子层上的非特异性吸附最小。DiynePE还用于将弹性体（聚二甲基硅氧烷：PDMS）制成的微结构附着在膜上，在两个表面之间形成一个受限的水溶液。微室使我们能够测定膜结合酶（cyochrome P450）的活性。通过光刻聚合DiynePC/DiynePE双层，将天然（流体）脂质双层与膜结合蛋白结合在一起。功能化聚合物双分子层和流体双分子层的杂交膜为广泛的生物医学应用提供了一个新的平台，包括生物传感器、生物测定、细胞培养和基于细胞的测定。
We describe a stable and functional model biological membrane based on a polymerized lipid bilayer with a chemically modified surface. A polymerized lipid bilayer was formed from a mixture of two diacetylene-containing phospholipids, 1,2-bis(10,12-tricosadiynoyl)-sn-glycero-3-phosphocholine (DiynePC) and 1,2-bis(10,12-tricosadiynoyl)-sn-glycero-3-phosphoethanolamine (DiynePE). DiynePC formed a stable bilayer structure, whereas the ethanolamine headgroup of DiynePE enabled functional molecules to be grafted onto the membrane surface. Copolymerization of DiynePC and DiynePE resulted in a robust bilayer. Functionalization of the polymeric bilayer provided a route to a robust and biomimetic surface that can be linked with biomolecules, cells, and three-dimensional (3D) microstructures. Biotin and peptides were grafted onto the polymeric bilayer for attaching streptavidin and cultured mammalian cells by molecular recognition, respectively. Nonspecific adsorption of proteins and cells on polymeric bilayers was minimum. DiynePE was also used to attach a microstructure made of an elastomer (polydimethylsiloxan: PDMS) onto the membrane, forming a confined aqueous solution between the two surfaces. The microcompartment enabled us to assay the activity of a membrane-bound enzyme (cyochrome P450). Natural (fluid) lipid bilayers were incorporated together with membrane-bound proteins by lithographically polymerizing DiynePC/DiynePE bilayers. The hybrid membrane of functionalized polymeric bilayers and fluid bilayers offers a novel platform for a wide range of biomedical applications including biosensor, bioassay, cell culture, and cell-based assay.