Water deprivation and rat adrenal mRNAs for tyrosine hydroxylase and the norepinephrine transporter.
Water deprivation and rat adrenal mRNAs for tyrosine hydroxylase and the norepinephrine transporter.
复制标题
缺水与大鼠肾上腺酪氨酸羟化酶和去甲肾上腺素转运蛋白的 mRNA。
DOI：
10.1152/ajpregu.1997.272.6.r1897
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Engeland,WC
中科院分区：
文献类型：
--
作者：
Brooks,VL;Huhtala,TA;Silliman,TL;Engeland,WC
Experiments were performed in rats to test the hypothesis that adrenal mRNA levels of tyrosine hydroxylase (TH) and the norepinephrine transporter (NET) would be modified by water deprivation via activation of the sympathetic nervous system. TH and NET mRNA levels were measured using the ribonuclease protection assay. Adrenal TH mRNA was higher (P < 0.001) in water-deprived (921 +/- 39 fg/microgram total RNA) compared with the water-replete rats (657 +/- 45 fg/microgram total RNA). In contrast, water deprivation decreased (P < 0.01) adrenal NET mRNA levels (275 +/- 66 vs. 433 +/- 63 fg/microgram total RNA). The dehydration-induced increase in TH mRNA was prevented by prior splanchnicectomy, but the decrease in NET mRNA was produced even in the absence of adrenal nerves. Water deprivation also increased (P < 0.05) plasma adrenocorticotropic hormone (84 +/- 16 vs. 42 +/- 14 pg/ml) and corticosterone (358 +/- 87 vs. 44 +/- 15 ng/ml) levels. Interestingly, the corticosterone response was reduced (P < 0.05) by unilateral adrenal denervation. These results suggest that water deprivation increases both adrenal medullary and adrenocortical activity at least in part by stimulation of sympathetic nerve activity.
