Therapeutic nanoreactors:: Combining chemistry and biology in a novel triblock copolymer drug delivery system
Therapeutic nanoreactors:: Combining chemistry and biology in a novel triblock copolymer drug delivery system
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DOI：
10.1021/nl051523d
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发表时间：
2005-11-01
期刊：
影响因子：
10.8
通讯作者：
Van Gelder, P
中科院分区：
文献类型：
--
作者：
Ranquin, A;Versées, W;Van Gelder, P
介绍了三嵌段共聚纳米反应器作为脂质体的替代方案，作为前药激活酶的包封载体。间日锥虫肌苷-腺苷-鸟苷首选核苷水解酶是一种潜在的前药激活酶，被包裹在聚（2-甲基氯唑啉）-聚（二甲基硅氧烷）-嵌段-（2-甲基氯唑啉）三嵌段共聚物构建的纳米大小的囊泡中。纳米反应器通过在反应器壁上掺入细菌孔蛋白（OmpF或Tsx）来实现功能化。证明了纳米反应器对三种天然底物和一种前药2-氟腺苷的有效裂解。
Triblock copolymeric nanoreactors are introduced as an alternative for liposomes as encapsulating carrier for prodrug activating enzymes. Inosine-adenosine-guanosine preferring nucleoside hydrolase of Trypanosoma vivax, a potential prodrug activating enzyme, was encapsulated in nanometer-sized vesicles constructed of poly(2-methyloxazoline)-block-poly(dimethylsiloxane)-block-(2-methyloxazoline) triblock copolymers. The nanoreactor is functionalized by incorporation of bacterial porins, OmpF or Tsx, in the reactor wall. Efficient cleavage of three natural substrates and one prodrug, 2-fluoroadenosine, by the nanoreactors was demonstrated.