Rhizosphere bacteria assembly derived from fumigation and organic amendment triggers the direct and indirect suppression of tomato bacterial wilt disease
Rhizosphere bacteria assembly derived from fumigation and organic amendment triggers the direct and indirect suppression of tomato bacterial wilt disease
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DOI：
10.1016/j.apsoil.2019.103364
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发表时间：
2020-03-01
影响因子：
4.8
通讯作者：
Shen, Qirong
中科院分区：
文献类型：
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作者：
Deng, Xuhui;Zhang, Na;Shen, Qirong
由于没有广泛认可的有效方法来控制或预防番茄青枯病（一种由土传细菌青枯菌引起的破坏性疾病），番茄生产受到严重阻碍。因此，我们的目标是提供一种基于使用碳酸氢铵熏蒸和有机改良剂来降低疾病严重程度的策略，并使用高通量测序评估这种处理对土壤微生物组的影响以及导致疾病抑制的潜在机制。结果表明，尽管在所有处理中，大块土壤和根际土壤中青枯菌的丰度很高，但这种组合策略仍能有效控制番茄青枯病。这种处理导致土壤细菌和真菌群落发生显着变化，在收获时，熏蒸和有机改良同样影响根际微生物组的变化。此外，根际细菌群落的变化是直接和间接抑制青枯菌和控制青枯病的关键因素。此外，根际的红细菌属、土单胞菌属和几丁噬菌属是参与疾病抑制的潜在类群。熏蒸和有机改良都通过降低青枯菌的丰度并改变细菌组成并结合刺激关键细菌类群来抑制疾病。
With no efficient method widely recognized for controlling or preventing tomato bacterial wilt, a devastating disease caused by the soil-borne bacterium Ralstonia solanacearum, tomato production is severely hindered. Therefore, the objective was thus to provide a strategy based on fumigation using ammonium bicarbonate along with organic amendment to reduce disease severity, and the impact of this treatment on the soil microbiome as well as the underlying mechanism leading to disease suppression were evaluated using high-throughput sequencing. Results showed that this combined strategy effectively controls tomato bacterial wilt disease despite the high abundance of R. solanacearum in both the bulk and rhizosphere soil in all treatments. The treatment led to significant changes in the soil bacterial and fungal communities, and at harvest time, fumigation and organic amendment equally affected the variation in the rhizosphere microbiome. Further, a shift in the rhizosphere bacterial community acted as the key factor directly and indirectly suppressing R. solanacearum and controlling bacterial wilt disease. In addition, Rhodanobacter, Terrimonas and Chitinophaga in the rhizosphere were the potential taxa involved in disease suppression. Both fumigation and organic amendment contributed to disease suppression by decreasing the abundance of R. solanacearum and altering the bacterial composition in combination with the stimulation of key bacterial taxa.