Peroxisomal ACBD4 interacts with VAPB and promotes ER-peroxisome associations.
Peroxisomal ACBD4 interacts with VAPB and promotes ER-peroxisome associations.
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DOI：
10.1080/15384101.2017.1314422
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发表时间：
2017-06-03
期刊：
影响因子：
--
通讯作者：
Schrader M
中科院分区：
文献类型：
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作者：
Costello JL;Castro IG;Schrader TA;Islinger M;Schrader M
线粒体、过氧化物酶体和内质网等细胞器之间的合作对于各种重要且多样化的代谢过程至关重要。有效的通讯和代谢物交换需要细胞器之间的物理连接，主要以细胞器接触位点的形式。在这些接触位点，细胞器膜通过分子系链的作用而紧密靠近，分子系链通常由锚定在相对细胞器膜上的特定蛋白质对组成。目前已鉴定出许多将 ER 与多种其他细胞器连接起来的束缚组分，但对将 ER 与过氧化物酶体连接的因素的了解有限。过氧化物酶体-ER相互作用很重要，因为它是不饱和脂肪酸、醚磷脂和甾醇生物合成所必需的，这些功能的缺陷会导致严重的疾病。在这里，我们将酰基辅酶A结合域蛋白4（ACBD4）描述为一种尾部锚定的过氧化物酶体膜蛋白，它与ER蛋白、囊泡相关膜蛋白相关蛋白B（VAPB）相互作用以促进过氧化物酶体-ER关联。
Cooperation between cellular organelles such as mitochondria, peroxisomes and the ER is essential for a variety of important and diverse metabolic processes. Effective communication and metabolite exchange requires physical linkages between the organelles, predominantly in the form of organelle contact sites. At such contact sites organelle membranes are brought into close proximity by the action of molecular tethers, which often consist of specific protein pairs anchored in the membrane of the opposing organelles. Currently numerous tethering components have been identified which link the ER with multiple other organelles but knowledge of the factors linking the ER with peroxisomes is limited. Peroxisome-ER interplay is important because it is required for the biosynthesis of unsaturated fatty acids, ether-phospholipids and sterols with defects in these functions leading to severe diseases. Here, we characterize acyl-CoA binding domain protein 4 (ACBD4) as a tail-anchored peroxisomal membrane protein which interacts with the ER protein, vesicle-associated membrane protein-associated protein–B (VAPB) to promote peroxisome-ER associations.