Evaluation of the Laser-Induced Thermotherapy Treatment Effect of Breast Cancer Based on Tissue Viscoelastic Properties
Evaluation of the Laser-Induced Thermotherapy Treatment Effect of Breast Cancer Based on Tissue Viscoelastic Properties
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基于组织粘弹性特性评价乳腺癌激光热疗治疗效果
DOI：
10.1115/1.4041502
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Hong Shan
中科院分区：
文献类型：
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作者：
Jiayao Chen;Bin Zhou;Suhao Qiu;Shengyuan Ma;Chung-Hao Lee;Ankush Aggarwal;Jianfeng Zeng;Mingyuan Gao;Yuan Feng;Dan Li;Hong Shan
光热疗法（PTT）已成为治疗癌症的有效，微创方法。然而，需要一种方法来定量评估激光诱导热疗（LITT）后的治疗效果。在这项研究中，我们使用三种不同功率密度的808 nm激光辐射来治疗小鼠模型中4 T1细胞系的乳腺癌组织。经处理的癌组织的粘弹性能的特征在于通过使用斜坡保持压痕法的两项Prony系列。我们观察到，瞬时剪切模量是显着高于未经处理的组织时，处理的癌组织与功率密度为1.5 W/cm ~ 2，但显着低于功率密度为2.5 W/cm ~ 2。在1.5 W/cm 2时，癌组织的长期剪切模量也显著高于未处理组织。通过末端脱氧核苷酸转移酶dUTP缺口末端标记法（TUNEL）评估细胞凋亡率来验证治疗效果。我们的研究结果表明，组织的粘弹性可能被用作生物标志物，用于评估LITT治疗效果。此外，我们还观察到治疗后的癌组织的应变无关行为，这为应用体内成像方法（如磁共振弹性成像（MRE））进行基于生物力学特性的治疗评价提供了有用的信息。
Photothermal therapy (PTT) has been emerging as an effective, minimally invasive approach to treat cancers. However, a method to quantitatively evaluate the treatment effect after laser-induced thermotherapy (LITT) is needed. In this study, we used 808 nm laser radiation with three different power densities to treat the breast cancer tissue from 4T1 cell lines in a mouse model. The viscoelastic properties of the treated cancer tissues were characterized by a two-term Prony series using a ramp-hold indentation method. We observed that instantaneous shear moduluswas significantly higher for the treated cancer tissues than that of the untreated tissue when treated with a power density of 1.5 W/cm2, but significantly lower with a power density of 2.5 W/cm2. The long-term shear moduluswas also significantly higher for the cancer tissue at 1.5 W/cm2, compared to the untreated tissue. The treatment effects were verified by estimating the cell apoptosis rate using terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL). Our results indicate that the viscoelastic properties of the tissue could potentially be used as biomarkers for evaluating the LITT treatment effect. In addition, we also observed a strain-independent behavior of the treated cancer tissue, which provided useful information for applying in vivo imaging method such as magnetic resonance elastography (MRE) for treatment evaluation based on biomechanical properties.