Dissecting the genetic architecture of waterlogging stress-related traits uncovers a key waterlogging tolerance gene in maize
Dissecting the genetic architecture of waterlogging stress-related traits uncovers a key waterlogging tolerance gene in maize
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DOI：
10.1007/s00122-018-3152-0
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发表时间：
2018-07
影响因子：
5.4
通讯作者：
F. Yu;Kun Liang;Zuxin Zhang;D. Du;Xuehai Zhang;Hailiang Zhao;Basir UI haq;F. Qiu
中科院分区：
文献类型：
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作者：
F. Yu;Kun Liang;Zuxin Zhang;D. Du;Xuehai Zhang;Hailiang Zhao;Basir UI haq;F. Qiu
关键信息通过全基因组关联分析，在16个与玉米耐涝性相关的遗传位点中检测到一个关键候选基因GRMZM 2G 110141，该基因可用于玉米分子标记辅助选择。然而，作物耐涝性的遗传基础在很大程度上是未知的。在这里，我们确定了耐涝相关性状的遗传位点进行全基因组关联研究，利用玉米表型评价在温室内涝胁迫和正常条件下。共鉴定出110个性状-单核苷酸多态性关联，跨越16个基因组区域，单个关联解释表型方差的2.88-10.67%。在确定的基因组区域中，有14个与先前检测到的耐涝相关的定量跟踪位点共定位。此外，33个候选基因参与了广泛的压力反应途径进行了预测。我们对138个随机选择的自交系中的一个关键候选基因（GRMZM 2G 110141）进行了重测序，发现在淹水条件下该基因的5 ′-UTR和mRNA丰度的变化与叶片损伤显著相关。此外，我们还检测了该基因的有利等位基因，并在两个不同的重组自交系群体中验证了有利等位基因。这些等位基因增强了分离群体的耐涝性，强烈表明GRMZM 2G 110141是一个关键的耐涝基因。这些耐涝相关的基因组区域和标记对玉米耐涝基因的分离和耐涝性状的改良具有重要意义。
Key messageA key candidate gene, GRMZM2G110141, which could be used in marker-assisted selection in maize breeding programs, was detected among the 16 genetic loci associated with waterlogging tolerance identified through genome-wide association study.AbstractWaterlogging stress seriously affects the growth and development of upland crops such as maize (Zea maysL.). However, the genetic basis of waterlogging tolerance in crop plants is largely unknown. Here, we identified genetic loci for waterlogging tolerance-related traits by conducting a genome-wide association study using maize phenotypes evaluated in the greenhouse under waterlogging stress and normal conditions. A total of 110 trait-single nucleotide polymorphism associations spanning 16 genomic regions were identified; single associations explained 2.88–10.67% of the phenotypic variance. Among the genomic regions identified, 14 co-localized with previously detected waterlogging tolerance-related quantitative trail loci. Furthermore, 33 candidate genes involved in a wide range of stress-response pathways were predicted. We resequenced a key candidate gene (GRMZM2G110141) in 138 randomly selected inbred lines and found that variations in the 5ʹ-UTR and in the mRNA abundance of this gene under waterlogging conditions were significantly associated with leaf injury. Furthermore, we detected favorable alleles of this gene and validated the favorable alleles in two different recombinant inbred line populations. These alleles enhanced waterlogging tolerance in segregating populations, strongly suggesting that GRMZM2G110141 is a key waterlogging tolerance gene. The set of waterlogging tolerance-related genomic regions and associated markers identified here could be valuable for isolating waterlogging tolerance genes and improving this trait in maize.