Impaired local microvascular vasodilatory effects of insulin and reduced skin microvascular vasomotion in obese women
Impaired local microvascular vasodilatory effects of insulin and reduced skin microvascular vasomotion in obese women
复制标题
DOI：
10.1016/j.mvr.2007.08.001
复制
发表时间：
2008-03-01
影响因子：
3.1
通讯作者：
Stehouwer, Coen D. A.
中科院分区：
文献类型：
--
作者：
de Jongh, Renate T.;Serne, Erik H.;Stehouwer, Coen D. A.
我们的研究目的是调查肥胖是否以胰岛素介导的血管舒张作用受损和皮肤微血管系统基础血管舒缩改变为特征。其中包括四十名健康肥胖女性和四十名健康瘦女性。通过阴极离子电渗疗法结合激光多普勒血流测定法测量胰岛素与对照物质相比的微血管效应。通过静息时皮肤激光多普勒血流的傅立叶变换分析来检查血管运动。与对照物质相比，局部施用胰岛素在瘦女性中诱导微血管舒张反应（中位值（四分位距）：31.6（17.1-43.9）与22.9（16.4-36.7）灌注单位，P = 0.04），但在肥胖女性中则没有诱导微血管舒张反应（28.1（14.4-47.1）与27.5） (17.5-48.2) 灌注单位，P = 0.7)。瘦女性中相对胰岛素诱导的血流量增加（针对对照物质进行校正）高于肥胖女性（重复测量的方差分析 F = 3.93，P = 0.05）。肥胖女性的总频谱 0.01-1.6 Hz 以及频率间隔 0.01-0.02 Hz 和 0.02-0.06 Hz（分别代表内皮和神经源性活动）对基础微血管血管舒缩的贡献低于瘦女性（所有 P < 0.05）。这些发现表明，肥胖的特征是胰岛素的直接微血管舒张作用受损，以及皮肤微血管血管舒缩减少，这提示内皮和神经源性活动发生改变。 (c) 2007 Elsevier Inc. 保留所有权利。
Our study aim is to investigate whether obesity is characterized by an impairment of insulin-mediated vasodilatory effects and by a modification of basal vasomotion in the skin microvasculature. Forty healthy obese and forty healthy lean women were included. Microvascular effects of insulin as compared to a control substance were measured by cathodal iontophoresis combined with laser Doppler flowmetry. Vasomotion was examined by Fourier transform analyses of skin laser Doppler flow at rest. Locally administered insulin, as compared to the control substance, induced a microvascular vasodilatory response in lean (median (interquartile range): 31.6 (17.1-43.9) vs. 22.9 (16.4-36.7) perfusion units, P = 0.04), but not in obese women (28.1 (14.4-47.1) vs. 27.5 (17.5-48.2) perfusion units, P = 0.7). The relative insulin-induced increase in blood flow corrected for the control substance was higher in lean than obese women (ANOVA for repeated measures F = 3.93, P = 0.05). The contribution of the total frequency spectrum 0.01-1.6 Hz and of the frequency intervals 0.01-0.02 Hz and 0.02-0.06 Hz (representative of endothelial and neurogenic activity, respectively) to basal microvascular vasomotion was lower in obese than in lean women (P < 0.05 for all). These findings show that obesity is characterized by an impaired direct microvascular vasodilatory effect of insulin and by decreased skin microvascular vasomotion in a way that is suggestive for alterations of endothelial and neurogenic activity. (c) 2007 Elsevier Inc. All rights reserved.