Fed-batch semi-simultaneous saccharification and fermentation of reed pretreated with liquid hot water for bio-ethanol production using Saccharomyces cerevisiae
Fed-batch semi-simultaneous saccharification and fermentation of reed pretreated with liquid hot water for bio-ethanol production using Saccharomyces cerevisiae
复制标题
用液体热水预处理的芦苇进行补料半同步糖化和发酵，利用酿酒酵母生产生物乙醇
DOI：
10.1016/j.biortech.2013.07.007
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发表时间：
2013-09-01
影响因子：
11.4
通讯作者：
Qu, Yinbo
中科院分区：
文献类型：
--
作者：
Lu, Jie;Li, Xuezhi;Qu, Yinbo
以芦苇为原料，采用液态热水预处理后进行流加半同步糖化发酵，以获得较高的酒精浓度和产量。结果表明，在180和210 ℃下预处理芦苇产生的水不溶性固体（WIS）可以有效地转化为乙醇的酿酒酵母。最佳发酵条件为：发酵温度36 ℃，pH 4.8，纤维素酶用量40滤纸活性单位/g烘干的葡萄糖，预水解温度50 ℃，预水解时间18 h。在18小时酶促预水解的6小时后加入约6.4%（w/v）补料分批底物。最高乙醇浓度达到39.4 g/L。在WIS中葡聚糖转化为乙醇的转化率分别达到79.1%（180 ℃）和75.1%（210 ℃）。每千克烘干芦苇的乙醇产率在180 ℃下为283 g/L，在210 ℃下为244 g/L。(C)2013爱思唯尔有限公司版权所有。
Reed was pretreated with liquid hot water (LHW) and then subjected to fed-batch semi-simultaneous saccharification and fermentation (S-SSF) to obtain high ethanol concentration and yield. Results show that water-insoluble solid (WIS) produced from reed pretreated at 180 and 210 degrees C could be effectively converted to ethanol by using Saccharomyces cerevisiae. The optimum conditions for bio-ethanol production are as follows: fermentation temperature of 36 degrees C, pH of 4.8 with cellulase loading of 40 filter paper activity units/g oven-dried WIS, and 18 h pre-hydrolysis at 50 degrees C. Approximately 6.4% (w/v) fed-batch substrate was added after 6 h of the 18 h enzymatic pre-hydrolysis. The highest ethanol concentration of 39.4 g/L was achieved. The conversion of glucan in the WIS to ethanol reached 79.1% (180 degrees C) and 75.1% (210 degrees C) respectively. The ethanol yields per kg of oven-dried reed were 283 g/L at 180 degrees C and 244 g/L at 210 degrees C. (C) 2013 Elsevier Ltd. All rights reserved.