Rad61/Wpl1 (Wapl), a cohesin regulator, controls chromosome compaction during meiosis.
Rad61/Wpl1 (Wapl), a cohesin regulator, controls chromosome compaction during meiosis.
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DOI：
10.1093/nar/gkw034
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发表时间：
2016-04-20
影响因子：
14.9
通讯作者：
Shinohara A
中科院分区：
文献类型：
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作者：
Challa K;Lee MS;Shinohara M;Kim KP;Shinohara A
减数分裂特异性粘连蛋白是连接姐妹染色单体所需的，在减数分裂前期 I 期间的各种染色体事件中发挥着关键作用，例如染色体形态发生和动力学以及重组。 Rad61/Wpl1（其他生物体中的 Wapl）负向调节粘连蛋白功能。在这项研究中，我们发现出芽酵母 rad61/wpl1 突变体的减数分裂染色体轴缩短，表明 Rad61/Wpl1 负向调节染色体轴压缩。减数分裂 I 期间端粒聚类的有效解析需要 Rad61/Wpl1，这表明 Rad61/Wpl1 对端粒动力学所需的粘连蛋白功能具有积极作用。此外，我们还证明了 Rad61/Wpl1 在减数分裂重组过程中的独特活性，包括其对中间体有效加工的影响。因此，Rad61/Wpl1 在减数分裂过程中正向和负向调节各种粘连蛋白介导的染色体过程。
Meiosis-specific cohesin, required for the linking of the sister chromatids, plays a critical role in various chromosomal events during meiotic prophase I, such as chromosome morphogenesis and dynamics, as well as recombination. Rad61/Wpl1 (Wapl in other organisms) negatively regulates cohesin functions. In this study, we show that meiotic chromosome axes are shortened in the budding yeast rad61/wpl1 mutant, suggesting that Rad61/Wpl1 negatively regulates chromosome axis compaction. Rad61/Wpl1 is required for efficient resolution of telomere clustering during meiosis I, indicating a positive effect of Rad61/Wpl1 on the cohesin function required for telomere dynamics. Additionally, we demonstrate distinct activities of Rad61/Wpl1 during the meiotic recombination, including its effects on the efficient processing of intermediates. Thus, Rad61/Wpl1 both positively and negatively regulates various cohesin-mediated chromosomal processes during meiosis.