MOLECULAR-CLONING OF HUMAN AND RAT COMPLEMENTARY-DNA ENCODING ANDROGEN RECEPTORS
MOLECULAR-CLONING OF HUMAN AND RAT COMPLEMENTARY-DNA ENCODING ANDROGEN RECEPTORS
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DOI：
10.1126/science.3353726
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发表时间：
1988-04-15
期刊：
影响因子：
56.9
通讯作者：
LIAO, SS
中科院分区：
文献类型：
--
作者：
CHANG, CS;KOKONTIS, J;LIAO, SS
从人睾丸和大鼠前列腺腹侧c DNA文库中获得编码雄激素受体的互补DNA(CDNA)。从cDNA的核苷酸序列推导出的氨基酸序列表明，存在一个在所有类固醇受体中高度保守的富含半胱氨酸的DNA结合域。人类的cDNA被转录，RNA产物在无细胞系统中被翻译，产生一个76千道尔顿的蛋白质。该蛋白可被人类雄激素受体的自身免疫抗体免疫沉淀。该蛋白与雄激素特异性结合，具有高亲和力。
Complementary DNAs (cDNAs) encoding androgen receptors were obtained from human testis and rat ventral prostate cDNA libraries. The amino acid sequence deduced from the nucleotide sequences of the cDNAs indicated the presence of a cysteine-rich DNA-binding domain that is highly conserved in all steroid receptors. The human cDNA was transcribed and the RNA product was translated in cell-free systems to yield a 76-kilodalton protein. The protein was immunoprecipitable by human autoimmune antibodies to the androgen receptor. The protein bound androgens specifically and with high affinity.