Isolation and characterization of two APETALA1-Like genes from mango (Mangifera indica L.)
Isolation and characterization of two APETALA1-Like genes from mango (Mangifera indica L.)
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芒果 (Mangifera indica L.) 中两个 APETALA1 样基因的分离和表征
DOI：
10.1016/j.scienta.2019.108814
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发表时间：
2020
影响因子：
4.3
通讯作者：
He Xinhua
中科院分区：
文献类型：
--
作者：
Yu Haixia;Luo Cong;Fan Yan;Zhang Xiujuan;Huang Fang;Li Mei;He Xinhua
APETALA1(AP1) encodes a key MADS-box transcription factor that plays a crucial role in the floral transition and floral organ development. Here we describe twoAP1homologues geneMiAP1-1andMiAP1-2from mango (Mangifera indicaL.). The full-lengthMiAP1-1andMiAP1-2were 960 bp and 906 bp, respectively, including 741 bp and 744 bp open reading frame encoding 247 and 248 amino acids, respectively.MiAP1-1andMiAP1-2exhibited high expression levels in floral organs and low transcript levels in vegetative organs.MiAP1-1was more highly expressed in leaves during the floral transition stage, whileMiAP1-2was more highly expressed in leaves during the floral transition stage, floral differentiation stage and inflorescence elongation stage. Overexpression ofMiAP1-1andMiAP1-2inArabidopsisresulted in significant early flowering, meanwhileMiAP1-2in transgenic tobacco also promote early flowering. Ectopic expression ofMiAP1-1andMiAP1-2causes different flower phenotypes.MiAP1-1caused the side branch flower organs mutate, whileMiAP1-2produced terminal flowers in the main stem shoots and also in the side branch. Several floral integrator genes were upregulated in the overexpression transgenic plant lines. These observations indicated that the twoMiAP1homologue genes in mango functions in the floral transition and floral organ development.
