Is the Difference in α-Amylase Activity in the Strains of Drosophila melanogaster with Different Allozymes Due to Transcriptional or Posttranscriptional Control?
Is the Difference in α-Amylase Activity in the Strains of Drosophila melanogaster with Different Allozymes Due to Transcriptional or Posttranscriptional Control?
复制标题
不同同位酶的果蝇品系中α-淀粉酶活性的差异是由转录或转录后控制引起的吗？
DOI：
10.1023/a:1018715528413
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发表时间：
1999
影响因子：
2.4
通讯作者：
T. Yamazaki
中科院分区：
文献类型：
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作者：
N. Yamate;T. Yamazaki
果蝇-淀粉酶在同工酶水平和比活性水平上都是一种高度多态性的酶。这种酶根据食物条件和发育阶段急剧改变其特定活性，并且已经提出根据食物来源改变其活性的能力与适应性呈正相关。但是，不同菌种和食物成分之间的特异性活性差异的原因尚不清楚。为了探讨这些差异的原因，我们在两种食物环境和两个发育阶段测量了8株具有不同电形态的黑腹d.m anogaster的淀粉酶比活性和Amy mRNA的相对量。我们发现了以下内容。首先，食物依赖性活性变化在转录水平上受到调控。菌株间特异性活性与mRNA水平呈显著相关。因此，80 - 90%的特异活性差异可以用mrna水平的差异来解释，但其余部分无法解释。菌株间和不同发育阶段的mRNA比活性均存在显著差异。这表明，每种同工酶的催化效率、菌株或阶段特异性翻译率、酶稳定性或两个Amy位点的差异使用都存在差异。
The alpha-amylase in Drosophilamelanogaster is a highly polymorphic enzyme, atboth the allozyme level and the specific activity level.This enzyme changes its specific activity drasticallydepending on both food conditions and developmental stages,and it has been suggested that the ability to change itsactivity depending on the source of food has positivecorrelation with fitness. But the cause of thedifference of specific activity among strainsand food compositions is not known. In order toinvestigate the cause of these differences, we measuredboth the specific activity of amylase and the relativeamount of Amy mRNA in eight strains of D.melanogaster with different electromorphs, in twofood environments and two developmental stages. We foundthe following. First, the food-dependent activity changeis regulated at the transcription level.Second, there was a significant correlation betweenspecific activity and mRNA level among strains. So 80 to90% of the specific activity difference can be explainedby differences in the level of mRNAs, but theremaining part cannot. Finally, there were significantdifferences in specific activity per mRNA both amongstrains and between developmental stages. This suggeststhat there are differences in the catalyticefficiency of each allozyme, strain- or stage-specifictranslation rate, enzyme stability, or differential useof two Amy loci.
H.Shibata：“果蝇物种亚群中重复的 Amy 基因座的分子进化：仅在编码区进行协同进化，并且在物种形成中存在过多的非同义取代。”
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