Voltage-activated sodium currents in acutely isolated mouse vestibular ganglion neurones
Voltage-activated sodium currents in acutely isolated mouse vestibular ganglion neurones
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DOI：
10.1097/00001756-199703240-00039
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发表时间：
1997-03-24
期刊：
影响因子：
1.7
通讯作者：
Desmadryl, G
中科院分区：
文献类型：
--
作者：
Chabbert, C;Chambard, JM;Desmadryl, G
使用膜片钳技术的全细胞配置研究从出生后小鼠中急性分离的前庭神经节神经元中的电压激活钠电流（I-Na）。在旨在允许 I-Na 去极化与 -80 mV 保持电位完全隔离的记录条件下，显示出快速失活的内向电流，该电流在 -60 mV 左右被激活，并在 -30 mV 左右表现出最大峰值电流。当用不含 Na+ 的溶液灌注细胞时，该电流被消除，并通过应用 100 nM 河豚毒素 (TTX) 几乎完全阻断。这些特性将这种内向电流识别为 TTX 敏感的 I-Na。 I-Na的半最大活化电位为-46 mV，其半最大失活电位为-69 mV。这是前庭初级神经元中电压激活钠电流的第一份报告。
VOLTAGE-ACTIVATED sodium currents (I-Na) in vestibular ganglion neurones acutely isolated from postnatal mice were investigated using the whole-cell configuration of the patch-clamp technique. Under recording conditions designed to allow the complete isolation of I-Na depolarizations from a holding potential of -80 mV revealed a fast inactivating inward current which was activated around -60 mV and exhibited maximum peak current around -30 mV. This current was eliminated when the cells were perifused with a Na+-free solution and almost totally blocked by application of 100 nM tetrodotoxin (TTX). These properties identify this inward current as TTX-sensitive I-Na. The half-maximum activation potential of I-Na was -46 mV and its half-maximum inactivation potential was -69 mV. This is the first report of voltage-activated sodium currents in vestibular primary neurones.