A DEOXYTHYMIDINE KINASE-DEFICIENT MUTANT OF ESCHERICHIA COLI .I. ISOLATION AND SOME PROPERTIES
A DEOXYTHYMIDINE KINASE-DEFICIENT MUTANT OF ESCHERICHIA COLI .I. ISOLATION AND SOME PROPERTIES
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DOI：
10.1016/0005-2787(67)90652-1
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发表时间：
1967-01-01
期刊：
影响因子：
--
通讯作者：
YURA, T
中科院分区：
文献类型：
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作者：
HIRAGA, S;IGARASHI, K;YURA, T
分离到一株不能将[2-14C]脱氧胸腺嘧啶纳入DNA的大肠杆菌菌株K12的新突变体（tdk-I），在不含脱氧胸腺嘧啶的培养基中生长正常。突变体缺乏脱氧胸腺嘧啶激酶（ATP：胸腺嘧啶5[image]-磷酸转移酶，EC 2.7.1.21）催化脱氧胸腺嘧啶转化为脱氧胸腺嘧啶5[image]-单磷酸的活性。突变体不能掺入5-溴脱氧尿嘧啶，即使培养基中存在尿嘧啶和脱氧胸苷，其生长也受到5-氟尿嘧啶或5-氟脱氧尿嘧啶的抑制。当突变细胞被T2或T4噬菌体感染时，观察到[2-14C]脱氧胸腺嘧啶的快速掺入，并伴有脱氧胸腺嘧啶激酶活性的出现。T4噬菌体感染诱导的酶可能与细菌脱氧胸苷激酶在质量上不同，T4噬菌体（可能还有其他T - even噬菌体）可能携带该酶的结构基因。
A new mutant (tdk-I) of E. coli strain K12 incapable of incorporating [2-14C]deoxythymidine into its DNA, while exhibiting normal growth in the medium without deoxythymidine, was isolated. The mutant lacks the activity of deoxythymidine kinase (ATP:thymidine 5[image]-phosphotransferase, EC 2.7.1.21) which catalyzes the conversion of deoxythymidine to deoxythymidine 5[image]-monophosphate. The mutant was unable to incoporate 5-bromo-deoxyuridine, and its growth was inhibited by 5-fluorouracil or 5-fluorodeoxyuridine even in the presence of uridine and deoxythymidine in the medium. When mutant cells were infected with T2 or T4 phage, a rapid incorporation of [2-14C]deoxythymidine accompanied by an appearance of deoxythymidine kinase activity was observed. The enzyme induced by infection with T4 phage may be qualitatively different from the bacterial deoxythymidine kinase, and T4 phage (and presumably other T even phages) may carry its own structural gene for this enzyme.