Development of a molecular marker associated with Verticillium wilt resistance in diploid interspecific potato hybrids
Development of a molecular marker associated with Verticillium wilt resistance in diploid interspecific potato hybrids
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DOI：
10.1007/s11032-008-9156-8
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发表时间：
2008-08-01
影响因子：
3.1
通讯作者：
Jansky, Shelley
中科院分区：
文献类型：
--
作者：
Bae, JinJoo;Halterman, Dennis;Jansky, Shelley
马铃薯黄萎病是由土传真菌大丽轮枝菌（Verticilliumdahliae）和白僵菌（V. albo-atrum）引起的一种严重病害。马铃薯中VW抗性的育种是具有挑战性的，这是由于模糊的症状表达、缺乏高通量筛选技术以及植物之间和植物内真菌定殖的可变性。遗传研究已经确定了二倍体茄（V-c）和种间杂种（V-w和V-t）中赋予抗性的主基因。然而，迄今为止，这些基因还没有被用于开发用于鉴定抗性克隆的分子标记。利用番茄Ve 1和Ve 2基因序列信息，从抗病和感病二倍体马铃薯杂交种中扩增出候选Ve基因的同源序列。产生CAPS标记以在抗性分离的回交群体中追踪VW抗性。该标记也在其他育种系中测试了其有用性。我们的研究结果表明，该标记是有效的选择V-w基因在分离育种群体。
Verticillium wilt (VW) is a widespread and serious potato (Solanum tuberosum) disease caused by the soilborne fungi Verticillium dahliae and V. albo-atrum. Breeding for VW resistance in potato is challenging due to ambiguous symptom expression, a lack of high throughput screening techniques, and variability in colonization by the fungus among and within plants. Genetic studies have identified major genes that confer resistance in diploid Solanum chacoense (V-c ) and interspecific hybrids (V-w and V-t ). However, to date, these genes have not been used to develop molecular markers for the identification of resistant clones. Tomato Ve1 and Ve2 gene sequence information was used to amplify candidate Ve gene orthologs from both resistant and susceptible diploid potato hybrids. A CAPS marker was generated to track VW resistance in a backcross population segregating for resistance. The marker was also tested for its usefulness in other breeding lines. Our results indicate that this marker is effective for selection of the V-w gene in segregating breeding populations.