Proteomic analyses of male contributions to honey bee sperm storage and mating.
Proteomic analyses of male contributions to honey bee sperm storage and mating.
复制标题
男性对蜜蜂精子储存和交配的贡献的蛋白质组学分析。
DOI：
10.1111/j.1365-2583.2006.00674.x
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发表时间：
2006-10
影响因子：
2.6
通讯作者：
Evans, J D
中科院分区：
文献类型：
--
作者：
Collins, A M;Caperna, T J;Williams, V;Garrett, W M;Evans, J D
蜜蜂（Apis mellifera L.）蚁后在生命早期交配并储存精子数年。雄性蜜蜂可能对精子的存活有很大的贡献。从成熟雄蜂的精囊和精液中提取蛋白质，通过电泳分离，并通过肽质量指纹图谱分析。对三个数据库，一般物种，蜜蜂和果蝇，进行了计算机搜索。光谱用于查询最近生成的蜜蜂基因组蛋白质列表以及一般物种和果蝇数据库。在发现的69种独特的蜜蜂蛋白中，有66种也存在于果蝇中。两种蛋白质只与蜜蜂基因匹配，一种是从苍蝇基因组中丢失的广泛蛋白质。糖酵解途径中涉及的基因过度表达。代谢相关蛋白主要存在于精囊中。在果蝇中鉴定的雄性附腺蛋白在蜜蜂中发现的蛋白质中很少有直系同源物。所选凝胶点的完整列表（包括蜜蜂基因组匹配和MALDI-TOF结果的Mascot搜索以及统计数据）见补充表。MALDI-TOF光谱和更完整的Mascot肽质量指纹图谱数据可应要求提供。补充图1-3显示了染色的蛋白质凝胶。
Honey bee (Apis mellifera L.) queens mate early in life and store sperm for years. Male bees likely contribute significantly to sperm survival. Proteins were extracted from seminal vesicles and semen of mature drones, separated by electrophoresis, and analysed by peptide mass fingerprinting. Computer searches against three databases, general species, honey bees and fruit flies, were performed. Spectra were used to query the recently generated honey bee genome protein list as well as general species and fruit fly databases. Of the 69 unique honey bee proteins found, 66 are also in Drosophila melanogaster. Two proteins only matched honey bee genes and one is a widespread protein lost from the fly genome. There is over-representation of genes implicated in the glycolysis pathway. Metabolism-associated proteins were found primarily in the seminal vesicle. Male accessory gland proteins as identified in Drosophila rarely had orthologs among proteins found in the honey bee. A complete listing of gel spots chosen including honey bee genome matches and Mascot searches of MALDI-TOF results with statistics is in the Supplementary table. MALDI-TOF spectra and more complete Mascot peptide mass fingerprinting data are available on request. Supplementary figs 1–3 show the stained protein gels.