Hybrid Nanocluster Plasmonic Resonator for Immunological Detection of Hepatitis B Virus
Hybrid Nanocluster Plasmonic Resonator for Immunological Detection of Hepatitis B Virus
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DOI：
10.1021/nn3034056
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发表时间：
2012-09-01
期刊：
影响因子：
17.1
通讯作者：
Chen, Fanqing Frank
中科院分区：
文献类型：
--
作者：
Draz, Mohamed Shehata;Fang, Binbin Amanda;Chen, Fanqing Frank
世界上大约88%的人口生活在乙肝病毒(乙肝病毒)中到高发病率的地区，然而目前的血清学和基于DNA的检测方法的灵敏度和便利性有限。在这里，我们描述了一种用于检测乙肝病毒的预组装等离子体共振纳米簇。带有乙肝病毒表面抗原(HBs Ag)表位的金纳米颗粒受体(AuNPs)和带有Fab抗体的量子点(QD)供体被组装成具有增强能量传递和荧光猝灭的免疫介导型3D导向复合体。Au和QD纳米粒子之间的相干等离子体共振被用来在纳米簇内实现改进的供体-受体共振，在存在乙肝病毒颗粒的情况下，纳米簇以高度特异的方式被分解。纳米簇提供了对乙肝病毒的高检测特异性和敏感度，敏感度限制在每微升1-100个病毒颗粒和全摩尔水平的乙肝表面抗原。该通用平台可用于建立多种其他微生物病原体的多重诊断方法。
Approximately 88% of the world population lives in regions with intermediate to high incidence of Hepatitis B virus (HBV), yet current serological and DNA-based detection methods have limited sensitivity and convenience. Here, we describe a preassembled plasmonic resonance nanocluster for HBV detection. The gold nanoparticle acceptors (AuNPs), with HBV surface antigen (HBsAg) epitope, and quantum dot (QD) donors with Fab antibody, are assembled into an immuno-mediated 3D-oriented complex with enhanced energy transfer and fluorescence quenching. The coherent plasmonic resonance between Au and QD nanoparticles is exploited to achieve improved donor-acceptor resonance within the nanocluster, which in the presence of HBV viral particles is disassembled in a highly specific manner. The nanocluster provides high detection specificity and sensitivity of HBV, with a sensitivity limit down to 1-100 viral particles per microliter and to attomolar levels of HBsAg. This general platform could be used to establish multiplex diagnostic assays for a variety of other microbial pathogens.