Nitrogen-Vacancy Magnetic Relaxometry of Nanoclustered Cytochrome C Proteins
Nitrogen-Vacancy Magnetic Relaxometry of Nanoclustered Cytochrome C Proteins
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DOI：
10.1021/acs.nanolett.3c03843
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发表时间：
2024-01-11
期刊：
影响因子：
10.8
通讯作者：
Laraoui，Abdelghani
中科院分区：
文献类型：
--
作者：
Lamichhane，Suvechhya;Timalsina，Rupak;Laraoui，Abdelghani
氮空位（NV）磁强计提供了一种检测神经元和细胞中铁水平的替代工具，具有良好的磁灵敏度和空间分辨率。本文采用NV-T1弛豫法检测细胞色素C （Cyt-C）纳米团簇中的铁。Cyt-C是一种含有单一血红素基团的水溶性蛋白，在线粒体的电子传递链中起着至关重要的作用。在环境条件下，血红素保持Fe+3顺磁性。我们在功能化金刚石芯片上进行NV-T1弛豫测量，并将Cyt-C的浓度从6 uM变化到54 uM，导致T1分别从1.2 ms减少到150 us。这种减少是由于Cyt-C中存在的铁自旋产生的自旋噪声。我们通过改变吸附铁的密度从1.44 × 10^6到1.7 × 10^7 / um^2，在纳米结构的金刚石芯片上对Cyt-C蛋白进行松弛成像。
Nitrogen-vacancy (NV) magnetometry offers an alternative tool to detect iron levels in neurons and cells with a favorable combination of magnetic sensitivity and spatial resolution. Here we employ NV-T1 relaxometry to detect Fe in cytochrome C (Cyt-C) nanoclusters. Cyt-C is a water-soluble protein that contains a single heme group and plays a vital role in the electron transport chain of mitochondria. Under ambient conditions, the heme group remains in the Fe+3 paramagnetic state. We perform NV-T1 relaxometry on a functionalized diamond chip and vary the concentration of Cyt-C from 6 uM to 54 uM, resulting in a decrease of T1 from 1.2 ms to 150 us, respectively. This reduction is attributed to spin-noise originating from the Fe spins present within the Cyt-C. We perform relaxometry imaging of Cyt-C proteins on a nanostructured diamond chip by varying the density of adsorbed iron from 1.44 x 10^6 to 1.7 x 10^7 per um^2.