Peristaltic pumps adapted for laminar flow experiments enhance in vitro modeling of vascular cell behavior.
Peristaltic pumps adapted for laminar flow experiments enhance in vitro modeling of vascular cell behavior.
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DOI：
10.1016/j.jbc.2022.102404
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发表时间：
2022-10
影响因子：
4.8
通讯作者：
Stratman, Amber N.
中科院分区：
文献类型：
--
作者：
Abello, Javier;Raghavan, Shreya;Yien, Yvette Y.;Stratman, Amber N.
内皮细胞（EC）是血管的主要细胞成分，在其生命周期中与血流动力学直接接触。在整个身体中，血管经历不同的血流模式和速率，从而改变血管结构和细胞行为。由于在完整的生物体中研究血流的复杂性，特别是在发育过程中，该领域越来越依赖于血流的体外建模作为研究内皮细胞中血液动力学依赖性信号传导机制的强大技术。虽然存在使流体再循环的商业流动系统，但许多市售泵是蠕动的，并且最佳地模拟脉动流动条件。然而，存在许多重要的情况，其中EC在体内经历层流条件，例如沿着脉管系统的沿着长的直线伸展。要了解EC功能在这些情况下，重要的是能够再现模型层流条件下，在体外。在这里，我们概述了一种方法，以可靠地适应市售蠕动泵研究层流条件。我们的概念验证研究侧重于2D模型，但可以进一步适应3D环境，以更好地模拟体内场景，例如器官发育。我们的研究在解决与流动建模相关的技术挑战方面取得了重大进展，并使我们能够进行功能研究，以了解剪切力对血管结构，细胞行为和重塑在不同生理环境中的机制作用。
Endothelial cells (ECs) are the primary cellular constituent of blood vessels that are in direct contact with hemodynamic forces over their lifetime. Throughout the body, vessels experience different blood flow patterns and rates that alter vascular architecture and cellular behavior. Because of the complexities of studying blood flow in an intact organism, particularly during development, the field has increasingly relied on in vitro modeling of blood flow as a powerful technique for studying hemodynamic-dependent signaling mechanisms in ECs. While commercial flow systems that recirculate fluids exist, many commercially available pumps are peristaltic and best model pulsatile flow conditions. However, there are many important situations in which ECs experience laminar flow conditions in vivo, such as along long straight stretches of the vasculature. To understand EC function under these contexts, it is important to be able to reproducibly model laminar flow conditions in vitro. Here, we outline a method to reliably adapt commercially available peristaltic pumps to study laminar flow conditions. Our proof-of-concept study focuses on 2D models but could be further adapted to 3D environments to better model in vivo scenarios, such as organ development. Our studies make significant inroads into solving technical challenges associated with flow modeling and allow us to conduct functional studies toward understanding the mechanistic role of shear forces on vascular architecture, cellular behavior, and remodeling in diverse physiological contexts.
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影响因子：
1.7
作者：
Hu, YL;Li, S;Chien, S
通讯作者：
Chien, S
影响因子：
7.7
作者：
Alghanem AF;Abello J;Maurer JM;Kumar A;Ta CM;Gunasekar SK;Fatima U;Kang C;Xie L;Adeola O;Riker M;Elliot-Hudson M;Minerath RA;Grueter CE;Mullins RF;Stratman AN;Sah R
通讯作者：
Sah R
DOI：
10.1083/jcb.201408103
发表时间：
2015-03-30
期刊：
The Journal of cell biology
影响因子：
--
作者：
Coon BG;Baeyens N;Han J;Budatha M;Ross TD;Fang JS;Yun S;Thomas JL;Schwartz MA
通讯作者：
Schwartz MA
DOI：
10.1152/ajpheart.01087.2006
发表时间：
2007-07-01
影响因子：
4.8
作者：
Himburg, Heather A.;Dowd, Scot E.;Friedman, Morton H.
通讯作者：
Friedman, Morton H.
影响因子：
16.6
作者：
Fang JS;Coon BG;Gillis N;Chen Z;Qiu J;Chittenden TW;Burt JM;Schwartz MA;Hirschi KK
通讯作者：
Hirschi KK