Antibiotic-DNA interactions: intermolecular nuclear Overhauser effects in the netropsin-d(C-G-C-G-A-A-T-T-C-G-C-G) complex in solution.
Antibiotic-DNA interactions: intermolecular nuclear Overhauser effects in the netropsin-d(C-G-C-G-A-A-T-T-C-G-C-G) complex in solution.
复制标题
抗生素-DNA 相互作用：溶液中的 netropsin-d(C-G-C-G-A-A-T-T-C-G-C-G) 复合物中的分子间核 Overhauser 效应。
DOI：
--
复制
发表时间：
1982
影响因子：
11.1
通讯作者：
D. Patel
中科院分区：
文献类型：
--
作者：
D. Patel
对d(C-G-C-G-A-A-T-T-C-G-C-C-G-G)双链及其Netropsin复合体的分子内核Overhaser效应(NOE)的测量结果表明，位于d(C-G-C-G-A-A-T-T-C-G-C-C-G)复合体的小槽中的质子标记是由同一碱基对和相邻碱基对上的可交换亚氨基质子和不可交换碱基质子组成的。根据Netropsin的吡咯环和侧链亚甲基质子与双链中心的腺苷H-2质子之间的分子间NOE效应，建立了抗生素凹面与十二核苷酸双链小槽d(A-A-T-T)四核苷酸片段之间的几个接触点。这些NOE测量为区分溶液中配体-DNA络合物中的次要和主要沟槽接触提供了一个强有力的方法。提出了双链DNA上网络蛋白酶在da X dt位点的相互作用模型。
The proton markers located in the minor groove of the d(C-G-C-G-A-A-T-T-C-G-C-G) duplex and its netropsin complex have been assigned from measurements of intramolecular nuclear Overhauser effects (NOEs) between exchangeable imino protons and nonexchangeable base protons on the same and adjacent base pairs. Several points of contact between the concave face of the antibiotic and the minor groove d(A-A-T-T) tetranucleotide segment of the dodecanucleotide duplex have been established based on intermolecular NOE effects between the pyrrole ring and side-chain methylene protons of netropsin and the adenosine H-2 protons of dA X dT base pairs in the center of the duplex. These NOE measurements provide a powerful method for differentiating between minor and major groove contacts in ligand-DNA complexes in solution. A model for netropsin interaction at dA X dT sites on duplex DNA is proposed.