Pathogenicity of Saprolegnia spp. to Atlantic salmon, Salmo salar L., eggs
Pathogenicity of Saprolegnia spp. to Atlantic salmon, Salmo salar L., eggs
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DOI：
10.1111/j.1365-2761.2011.01273.x
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发表时间：
2011-08-01
影响因子：
2.5
通讯作者：
Skaar, I.
中科院分区：
文献类型：
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作者：
Thoen, E.;Evensen, O.;Skaar, I.
用8个不同来源、已知形态特征和生长/萌发模式的不同菌株对活的和死的大西洋鲑鱼眼卵进行了挑战。还测试了一些菌株对大西洋鲑鱼Parr的致病性。通过暴露在悬浮液中的孢子或通过将活卵与受感染的死卵共同孵化来进行卵子的攻击。根据攻毒试验中观察到的致病力，对分离株的表型特征进行了评价，以确定可能的毒力因子，从而导致鸡腐病的卵子感染。实验结果证实，活卵对悬浮液中的腐霉孢子不易感染，活卵的感染只能从以死卵或碎片为代表的感染核发生。结果表明，鲑鱼PARR致病菌株对鱼卵的侵染性不强，对鱼卵的侵染率最高的菌株为腐生菌。
Live and dead Atlantic salmon eyed eggs were challenged with eight different Saprolegnia isolates, selected because of their varied origins, known morphological characteristics and growth/germination pattern. Some isolates were also tested for pathogenicity to Atlantic salmon parr. Challenge of eggs was performed by exposure to spores in suspension or by co-incubation of live eggs with infected dead eggs. The phenotypic characteristics of the isolates were evaluated in relation to their observed pathogenicity from the challenge experiment, to identify possible virulence factors leading to egg-infection by Saprolegnia. The results from the experiments confirm that live eggs are refractory to infection with Saprolegnia spores in suspension and that an infection of live eggs can only occur from an infection nucleus represented by dead eggs or debris. It was observed that strains pathogenic to salmon parr were not particularly infective towards eggs, and the isolates that gave the highest infection rates to eggs were species considered to be saprotrophs.