Determination of benzimidazole residues in animal tissue samples by combination of magnetic solid-phase extraction with capillary zone electrophoresis
Determination of benzimidazole residues in animal tissue samples by combination of magnetic solid-phase extraction with capillary zone electrophoresis
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磁力固相萃取结合毛细管区带电泳测定动物组织样品中苯并咪唑残留量
DOI：
10.1016/j.talanta.2011.12.038
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发表时间：
2012-01-30
期刊：
影响因子：
6.1
通讯作者：
Feng, Yu-Qi
中科院分区：
文献类型：
--
作者：
Hu, Xi-Zhou;Chen, Ming-Luan;Feng, Yu-Qi
苯并咪唑类药物（BZD）是一类广泛应用于食品动物防治的人工合成驱虫药。为保护消费者免受BZD残留的危害，建立了一种简便、快速、高效的同时测定动物组织中10种BZD残留的方法。该分析方法包括使用磁铁矿/二氧化硅/聚（甲基丙烯酸-co-乙二醇二甲基丙烯酸酯）（Fe 3 O 4/SiO2/聚（MAA-co-EGDMA））磁性微球，通过磁性固相萃取（MSPE）提取样品，并通过毛细管区带电泳（CZE）进行测定。为了提高方法的灵敏度，我们采用了电动进样场放大样品堆积技术（FASS）。以小檗碱溶液为内标，减少了分析结果的波动。在优化的提取条件下，10种BZD的线性关系良好，相关系数（R-2）均在0.9920以上。10种BZD在猪肉和猪肝中的检出限分别为1.05-10.42 ng/g和1.06-12.61 ng/g。方法的日内和日间相对标准偏差（RSD）均小于13.6%。10种BZD的加标回收率为81.1%~ 105.4%，相对标准偏差小于9.3%。(C)2011 Elsevier B. V.保留所有权利。
Benzimidazole drugs (BZDs) comprise a large number of synthetic anthelmintics, which are widely used in food-producing animals for prophylactic and therapeutic purposes. To protect consumers from the risks related to BZDs residues, a simple, rapid, and efficient method for simultaneous determination of ten BZDs in animal tissues samples was developed. This analytical procedure involved extracting samples with magnetic solid-phase extraction (MSPE) using magnetite/silica/poly (methacrylic acid-co-ethylene glycol dimethacrylate) (Fe3O4/SiO2/poly (MAA-co-EGDMA)) magnetic microspheres, and determination by capillary zone electrophoresis (CZE). To improve the sensitivity of the method, we employed the electrokinetic injection with field-amplified sample stacking technique (FASS). Berbine solution was used as internal standard to minimize the fluctuation of analytical results. Under the optimized extraction conditions, good linearities were obtained for the ten BZDs with the correlation coefficients (R-2) above 0.9920. The limits of detections (LODs) for ten BZDs were 1.05-10.42 ng/g in swine muscle and 1.06-12.61 ng/g in swine liver, respectively. The intra- and inter-day relative standard deviations (RSDs) of the developed method were less than 13.6%. The recoveries of the ten BZDs for the spiked samples ranged from 81.1% to 105.4% with RSDs less than 9.3%. (C) 2011 Elsevier B.V. All rights reserved.