THE SEQUENCE SPECIFICITY OF HOMEODOMAIN-DNA INTERACTION
THE SEQUENCE SPECIFICITY OF HOMEODOMAIN-DNA INTERACTION
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DOI：
10.1016/0092-8674(88)90123-7
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发表时间：
1988-09-23
期刊：
影响因子：
64.5
通讯作者：
OFARRELL, PH
中科院分区：
文献类型：
--
作者：
DESPLAN, C;THEIS, J;OFARRELL, PH
果蝇发育基因编码了序列特异性的DNA结合活性。利用在大肠杆菌中表达为融合蛋白的缺失构建体，我们将这种活性定位在保守同源结构域（HD）上。结合位点共识，TCAATTAAAT，被发现在包裹调控区域的簇中。En HD与一个合成共识副本的弱绑定被相邻副本增强。由fushi tarazu编码的远亲HD与En HD结合在相同的位点上，但其对相关位点的偏好不同。两种hd都结合第二种序列，即TAA的重复序列。En和Ftz hd序列特异性的相似性表明，在DNA结合蛋白家族中，近亲会表现出相似的特异性。相关调节蛋白之间的竞争可能决定了哪个蛋白占据了给定的结合位点，从而决定了顺化调节位点的最终效果。
The Drosophila development gene, engrailed, encodes a sequence-specific DNA binding activity. Using deletion constructs expressed as fusion proteins in Escherichia coli, we localized this activity to the conserved homeodomain (HD). The binding site consensus, TCAATTAAAT, is found in clusters in the engrailed regulatory region. Weak binding of the En HD to one copy of a synthetic consensus is enhanced by adjacent copies. The distantly related HD encoded by fushi tarazu binds to the same sites as the En HD, but differs in its preference for related sites. Both HDs bind a second type of sequence, a repeat of TAA. The similarity in sequence specificity of En and Ftz HDs suggests that, within families of DNA binding proteins, close relatives will exhibit similar specificities. Competition among related regulatory proteins might govern which protein occupies a given binding site and consequently determine the ultimate effect of cisacting regulatory sites.