Coherent anti-Stokes Raman scattering imaging under ambient light.
Coherent anti-Stokes Raman scattering imaging under ambient light.
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DOI：
10.1364/ol.41.003880
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发表时间：
2016-08-15
期刊：
影响因子：
3.6
通讯作者：
Cheng JX
中科院分区：
文献类型：
--
作者：
Zhang Y;Liao CS;Hong W;Huang KC;Yang H;Jin G;Cheng JX
我们展示了环境光相干反斯托克斯拉曼散射(AL-CARS)显微镜，它允许CARS成像在野外使用的环境光下操作。CARS信号以兆赫频率调制，由配备实验室构建的谐振放大器的光电二极管检测，然后通过锁定放大器提取。光谱域和频域的滤光片有效地阻挡了室内光线对汽车图像的污染。展示了在环境光下肿瘤组织的原位高光谱CARS成像。
We demonstrate ambient light coherent anti-Stokes Raman scattering (AL-CARS) microscopy that allows CARS imaging to be operated under environment light for field use. CARS signal is modulated at megahertz frequency and detected by a photodiode equipped with a lab-built resonant amplifier, then extracted through a lock-in amplifier. Filters in both spectral domain and frequency domain effectively blocked room light contamination of the CARS image. In situ hyperspectral CARS imaging of tumor tissue under ambient light is demonstrated.