Analysis of carbonylated proteins in bronchoalveolar lavage of patients with diffuse lung diseases
Analysis of carbonylated proteins in bronchoalveolar lavage of patients with diffuse lung diseases
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DOI：
10.1007/s00408-007-9001-6
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发表时间：
2007-05-01
期刊：
影响因子：
5
通讯作者：
Rottoli, Paola
中科院分区：
文献类型：
--
作者：
Bargagli, Elena;Penza, Francesco;Rottoli, Paola
弥漫性肺疾病（DLD）是一组具有不同病因、临床病程和预后的异质性疾病。研究表明，氧化应激可促进这些疾病的发病。在本研究中，我们测量了结节病、系统性硬化症相关肺纤维化、特发性肺纤维化患者BAL中的羰基化蛋白浓度，并首次测量了慢性嗜酸性粒细胞性肺炎和外源性过敏性肺泡炎患者BAL中的羰基化蛋白浓度。我们的目的是进一步研究弥漫性肺病中的氧化产物。与对照组相比，患者BAL中的氧化修饰蛋白浓度增加（0.22 nmol/mg蛋白vs 0.05 nmol/mg蛋白; p < 0.001），并且在每个疾病组与对照组中，这表明因氧化而功能障碍的蛋白质可能在弥漫性肺疾病的发病机制中起作用。需要对更多患者进行进一步研究，以了解氧化修饰蛋白对DLD发病机制的贡献，特别是对外源性过敏性肺泡炎的发展的贡献，其中发现了最高水平的羰基化蛋白。
Diffuse lung diseases (DLD) are a heterogeneous group of diseases with different etiopathogenesis, clinical course, and prognosis. It has been demonstrated that oxidative stress can contribute to the pathogenesis of these diseases. In the present study we measured carbonylated protein concentrations in the BAL of patients with sarcoidosis, pulmonary fibrosis associated with systemic sclerosis, idiopathic pulmonary fibrosis, and for the first time in patients with chronic eosinophilic pneumonia and extrinsic allergic alveolitis. Our aim was to further investigate oxidation products in diffuse lung diseases. Oxidatively modified protein concentrations were increased in the BAL of patients than in that of controls (0.22 nmol/mg protein vs 0.05 nmol/mg protein; p < 0.001) and in each group of disease versus controls, suggesting that proteins that have become dysfunctional by oxidation could play a role in the pathogenesis of diffuse lung diseases. Further studies in a greater number of patients are needed to understand the contribution of oxidatively modified proteins to the pathogenesis of DLD and, in particular, to the development of extrinsic allergic alveolitis where the highest levels of carbonylated proteins were found.