Isolation and characterization of human breast cancer cells overexpressing S-adenosylmethionine decarboxylase.
Isolation and characterization of human breast cancer cells overexpressing S-adenosylmethionine decarboxylase.
复制标题
DOI：
10.1016/0304-3835(95)03860-y
复制
发表时间：
1995-08
期刊：
影响因子：
9.7
通讯作者：
A. Manni;B. Badger;R. Grove;S. Kunselman;L. Demers
中科院分区：
文献类型：
--
作者：
A. Manni;B. Badger;R. Grove;S. Kunselman;L. Demers
我们报告的第一个成功的分离和S-腺苷甲硫氨酸脱羧酶（SAMDC）过表达细胞的初步表征使用转染方法。稳定转染的MCF-7乳腺癌细胞过度产生SAMDC（~5倍），表现出鸟氨酸脱羧酶减少，而N′-亚精胺/精胺乙酰转移酶水平急剧增加。细胞多胺谱分析表明，精胺选择性增加（~80%），而亚精胺和腐胺水平降低（~48%和~15%的控制，分别）。由于SAMDC过表达克隆在软琼脂中表现出克隆形成性增加，我们的数据表明，精胺可能选择性地参与赋予乳腺癌细胞更具侵袭性的表型。
We report the first successful isolation and initial characterization of S-adenosylmethionine decarboxylase (SAMDC)-overexpressing cells using a transfection approach. Stably transfected MCF-7 breast cancer cells overproducing SAMDC (~5-fold) manifested reduced ornithine decarboxylase while levels of N′-spermidine/spermine acetyltransferase were variably increased. Analysis of cellular polyamine profile showed that spermine was selectively increased (~80%), while spermidine and putrescine levels were reduced (~48% and ~15% of control, respectively). Since SAMDC-overexpressing clones exhibited increased clonogenicity in soft agar, our data suggest that spermine may be selectively involved in conferring a more invasive phenotype to breast cancer cells.