Cytoplasmic transfer of the mitogenic response to platelet-derived growth factor.
Cytoplasmic transfer of the mitogenic response to platelet-derived growth factor.
复制标题
对血小板衍生生长因子的促有丝分裂反应的细胞质转移。
DOI：
10.1073/pnas.78.7.4363
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发表时间：
1981
影响因子：
11.1
通讯作者：
Stiles,CD
中科院分区：
文献类型：
--
作者：
Smith,JC;Stiles,CD
BALB/c 3 T3小鼠细胞短暂暴露于血小板衍生生长因子（PDGF）变得“有能力”复制其DNA并分裂。当细胞用PDGF处理，然后与未处理的细胞融合时，产生的异核体能够复制它们的DNA。来源于PDGF处理的细胞的细胞质也能够将生长反应转移到未处理的细胞。在细胞质转移到另一个细胞后，PDGF诱导的促有丝分裂信号的强度被衰减，衰减的因子大致与总细胞质体积的增加成比例。当RNA合成在PDGF处理期间被阻断时，细胞不获得将PDGF生长信号传递到未处理细胞的能力。相比之下，在PDGF治疗期间暴露于放线菌酮没有影响。这些观察结果表明，细胞质转移的生长反应PDGF（能力）介导的PDGF诱导的稳定的RNA，而不是由PDGF本身或PDGF-受体复合物。PDGF-对照异核体中DNA合成的开始至少发生在细胞融合后11小时。因此，在这些细胞融合中转移的物质并不直接参与DNA合成;相反，它似乎触发了一系列最终导致DNA合成的事件。
BALB/c 3T3 mouse cells exposed briefly to platelet-derived growth factor (PDGF) become "competent" to replicate their DNA and divide. When cells are treated with PDGF and then fused to untreated cells, the resulting heterokaryons become competent to replicate their DNA. Cytoplasts derived from PDGF-treated cells are also able to transfer the growth response to untreated cells. After cytoplasmic transfer to another cell, the strength of the PDGF-induced mitogenic signal is attenuated by a factor roughly proportional to the increase in total cytoplasmic volume. When RNA synthesis is blocked during PDGF treatment, cells do not acquire the capacity to transfer the PDGF growth signal to untreated cells. By contrast, exposure to cycloheximide during PDGF treatment has no effect. These observations suggest that cytoplasmic transfer of the growth response to PDGF (competence) is mediated by a PDGF-induced stable RNA rather than by PDGF itself or a PDGF--receptor complex. The onset of DNA synthesis in PDGF--control heterokaryons occurs a minimum of 11 hr after cell fusion. Thus the substance that is transferred in these cell fusions is not directly involved in DNA synthesis; rather, it seems to trigger a sequence of events culminating in DNA synthesis.