Supplementation with branched-chain amino acids to a low-protein diet regulates intestinal expression of amino acid and peptide transporters in weanling pigs
Supplementation with branched-chain amino acids to a low-protein diet regulates intestinal expression of amino acid and peptide transporters in weanling pigs
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DOI：
10.1007/s00726-013-1577-y
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发表时间：
2013-11-01
期刊：
影响因子：
3.5
通讯作者：
Wu, Guoyao
中科院分区：
文献类型：
--
作者：
Zhang, Shihai;Qiao, Shiyan;Wu, Guoyao
本研究旨在确定日粮中支链氨基酸（AA）对采食低蛋白（LP）日粮断奶仔猪生长性能、空肠氨基酸及肽转运载体表达，以及结肠微生物菌群的影响。选用108头大白×长白×杜洛克仔猪（28日龄断奶），分别饲喂正常蛋白日粮（NP，粗蛋白20.9%）、低蛋白日粮（LP，粗蛋白17.1%）或添加支链氨基酸的低蛋白日粮（LP + BCAA，粗蛋白17.9%），试验期14天。日粮蛋白质限制降低了仔猪的生长性能和小肠绒毛高度，而在低蛋白日粮中添加支链氨基酸可使这些指标恢复至正常蛋白日粮组水平。采食低蛋白日粮仔猪血清中的支链氨基酸浓度降低，而采食LP + BCAA日粮仔猪的血清支链氨基酸浓度与正常蛋白日粮组相近。与采食低蛋白日粮的仔猪相比，采食LP + BCAA日粮仔猪的Na⁺-中性氨基酸交换载体2、阳离子氨基酸转运载体1、b(0,+)氨基酸转运载体和4F2重链的mRNA水平更高。然而，与采食低蛋白日粮的仔猪相比，采食LP + BCAA日粮仔猪的肽转运载体1的mRNA和蛋白水平更低。三组仔猪的结肠微生物菌群无差异。综上所述，在低蛋白日粮中添加支链氨基酸可提高断奶仔猪的生长性能、肠道发育及肠道氨基酸转运载体的表达。我们的研究结果为进一步认识支链氨基酸作为动物营养中功能性氨基酸提供了新的分子基础。
This study determined the effects of dietary branched-chain amino acids (AA) (BCAA) on growth performance, expression of jejunal AA and peptide transporters, and the colonic microflora of weanling piglets fed a low-protein (LP) diet. One hundred and eight Large White x Landrace x Duroc piglets (weaned at 28 days of age) were fed a normal protein diet (NP, 20.9 % crude protein), an LP diet (LP, 17.1 % crude protein), or an LP diet supplemented with BCAA (LP + BCAA, 17.9 % crude protein) for 14 days. Dietary protein restriction reduced piglet growth performance and small-intestinal villous height, which were restored by BCAA supplementation to the LP diet to values for the NP diet. Serum concentrations of BCAA were reduced in piglets fed the LP diet while those in piglets fed the LP + BCAA diet were similar to values for the NP group. mRNA levels for Na+-neutral AA exchanger-2, cationic AA transporter-1, b(0,+) AA transporter, and 4F2 heavy chain were more abundant in piglets fed the LP + BCAA diet than the LP diet. However, mRNA and protein levels for peptide transporter-1 were lower in piglets fed the LP + BCAA diet as compared to the LP diet. The colonic microflora did not differ among the three groups of pigs. In conclusion, growth performance, intestinal development, and intestinal expression of AA transporters in weanling piglets are enhanced by BCAA supplementation to LP diets. Our findings provide a new molecular basis for further understanding of BCAA as functional AA in animal nutrition.