Stimulation of cell proliferation and apoptosis in the presence of amino acids in organotypic culture of tissues of different degree of maturity.
Stimulation of cell proliferation and apoptosis in the presence of amino acids in organotypic culture of tissues of different degree of maturity.
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DOI：
10.1134/s0012496606010030
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发表时间：
2006-01-01
期刊：
影响因子：
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通讯作者：
Nozdrachev, A D
中科院分区：
文献类型：
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作者：
Chalisova, N I;Penniyainen, V A;Nozdrachev, A D
CHALISOVA等人的氨基酸序列为甘氨酸（Gly）、丙氨酸（Ala）、天冬酰胺（Asn）、组氨酸（His）、赖氨酸（Lys）、丝氨酸（Ser）、谷氨酰胺（Gln）、精氨酸（Arg）、脯氨酸（Pro）、天冬氨酸（Asp）、谷氨酸（Glu）、酪氨酸（Tyr）、半胱氨酸（Cys）、缬氨酸（瓦尔）、苏氨酸（Thr）、甲硫氨酸（Met）、亮氨酸（Leu）、异亮氨酸（Ile）、苯丙氨酸（Phe）、和色氨酸（Trp）。将培养皿在37 ℃下在恒温箱中孵育3天，然后使用配备有显微遥测目镜（series 10，MTN-13，Alfa-Telekom，Russia）的相差显微镜检查。对于每个外植体，我们确定面积指数（AI），其计算为外植体总面积（连同迁移细胞区）与外植体中心区面积之间的比率，并以任意单位表示。使用PhotoM 1.2软件计算外植体的AI值。
CHALISOVA et al. were glycine (Gly), alanine (Ala), asparagine (Asn), histidine (His), lysine (Lys), serine (Ser), glutamine (Gln), arginine (Arg), proline (Pro), aspartic acid (Asp), glutamic acid (Glu), tyrosine (Tyr), cysteine (Cys), valine (Val), threonine (Thr), methionine (Met), leucine (Leu), isoleucine (Ile), phenylalanine (Phe), and tryptophane (Trp). Petri dishes were incubated at 37 C for 3 days in a constant-temperature cabinet and then examined using a phase-contrast microscope equipped with a microtelemetric eyepiece (series 10, MTN-13, Alfa-Telekom, Russia). For each explant we determined the area index (AI), which was calculated as the ratio between the total area of explant (together with the zone of migrating cells) and the area of the central zone of explant and expressed in arbitrary units. The AI values of explants were calculated using the PhotoM 1.2 software.