Chemically Mediated Mechanical Expansion of the Pollen Tube Cell Wall
Chemically Mediated Mechanical Expansion of the Pollen Tube Cell Wall
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DOI：
10.1016/j.bpj.2011.08.016
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发表时间：
2011-10-19
影响因子：
3.4
通讯作者：
Dumais, Jacques
中科院分区：
文献类型：
--
作者：
Rojas, Enrique R.;Hotton, Scott;Dumais, Jacques
植物细胞的形态发生相当于围绕它们的坚硬细胞壁的成形。为此，这些细胞整合了两个伴随的过程：1）新材料沉积到现有的壁中，以及2）该材料通过膨压的机械变形。然而，由于协调这些过程的机制的不确定性，现有的模型通常采用一个限制的情况下，其中一个或另一个决定形态发生。在这份报告中，我们制定了一个简单的机制，在花粉管沉积引起营业额的细胞壁交联，从而促进机械变形。因此，沉积和力学是耦合的，都是形态发生过程的组成部分。该模型的关键实验资格是：它能够精确地再现花粉管的形态;它预测快速生长的花粉管所表现出的生长振荡;和它预测观察到的变量之间的相位关系，如壁厚，细胞形态，和振荡细胞内的生长速率。简而言之，该模型捕捉了丰富的现象学的花粉管形态发生，并对其他植物细胞类型的影响。
Morphogenesis of plant cells is tantamount to the shaping of the stiff cell wall that surrounds them. To this end, these cells integrate two concomitant processes: 1), deposition of new material into the existing wall, and 2), mechanical deformation of this material by the turgor pressure. However, due to uncertainty regarding the mechanisms that coordinate these processes, existing models typically adopt a limiting case in which either one or the other dictates morphogenesis. In this report, we formulate a simple mechanism in pollen tubes by which deposition causes turnover of cell wall cross-links, thereby facilitating mechanical deformation. Accordingly, deposition and mechanics are coupled and are both integral aspects of the morphogenetic process. Among the key experimental qualifications of this model are: its ability to precisely reproduce the morphologies of pollen tubes; its prediction of the growth oscillations exhibited by rapidly growing pollen tubes; and its prediction of the observed phase relationships between variables such as wall thickness, cell morphology, and growth rate within oscillatory cells. In short, the model captures the rich phenomenology of pollen tube morphogenesis and has implications for other plant cell types.