A simple and efficient method for the solid-phase extraction of dissolved organic matter (SPE-DOM) from seawater
A simple and efficient method for the solid-phase extraction of dissolved organic matter (SPE-DOM) from seawater
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DOI：
10.4319/lom.2008.6.230
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发表时间：
2008-06-01
影响因子：
2.7
通讯作者：
Kattner, Gerhard
中科院分区：
文献类型：
--
作者：
Dittmar, Thorsten;Koch, Boris;Kattner, Gerhard
提出了一种使用商业预装小柱从海水中固相萃取溶解有机物(SPE-DOM)的简单方法。该方法不需要主要的仪器设备，可以在现场进行。考虑了改性的二乙烯基苯聚合物型吸附剂(VARIAN PPL和ENV)和具有不同碳氢链键合的二氧化硅结构的吸附剂(VARIAN C8、C18、C18OH和C18EWP)。除了严重污染样品的C18OH外，所有的吸附剂都没有明显的溶解有机碳(DOC)或氮(DON)。来自巴西北部陆架的样品具有强烈的陆源和海洋DOM混合梯度，用来比较各种吸着剂。PPL是最有效的--平均而言，62%的DOC以无盐提取物的形式回收。在硅基脱色剂中，C18的效率最高，但它的提取效率仅为PPL的三分之二。[H-1]核磁共振、C/N和Delta C-13分析表明，PPL提取的DOM比例比C18更具代表性。因此，PPL被用于墨西哥湾和南极洲的比较研究。微咸水沼泽和河水中的DOC分别占总DOC的65%和62%。对于南极和深海的纯海洋DOM，提取效率较低(平均为43%)。新方法分离海洋DOM的效率优于或接近高劳动量的方法。另一个优点是样品完全脱盐。主要DOM组分的分离是无盐的，这为通过先进的分析技术进一步表征DOM提供了许多可能性。
A simple protocol is presented for the solid-phase extraction of dissolved organic matter (SPE-DOM) from seawater using commercially prepacked cartridges. The method does not require major instrumentation and can be performed in the field. Modified styrene divinyl benzene polymer type sorbents ( Varian PPL and ENV) and sorbents of a silica structure bonded with different hydrocarbon chains ( Varian C8, C18, C18OH, and C18EWP) were considered. Except for C18OH, which heavily contaminated the samples, none of the sorbents leached significant amounts of dissolved organic carbon ( DOC) or nitrogen ( DON). Samples from the North Brazil shelf with strong mixing gradients of terrigenous and marine DOM were used to compare the various sorbents. PPL was the most efficient - on average, 62% of DOC was recovered as salt-free extracts. C18 was found to be most efficient among the silica-based sorbents, but it showed only two- thirds of the extraction efficiency of PPL. As indicated by [ H-1] NMR, C/ N, and delta C-13 analyses, PPL extracted a more representative proportion of DOM than C18. Therefore, PPL was used for comparative studies in the Gulf of Mexico and Antarctica. From brackish marsh and river waters, 65% and 62% of total DOC, respectively, could be extracted. For purely marine DOM in Antarctica and the deep sea, the extraction efficiency was lower ( 43% on average). The efficiency of the new method to isolate marine DOM is better than or similar to highly laborious methods. A further advantage is the complete desalination of the sample. The isolation of a major DOM fraction, which is salt- free, offers many possibilities to further characterize DOM by advanced analytical techniques.