Novel synthesis of cerium oxide nanoparticles for free radical scavenging
Novel synthesis of cerium oxide nanoparticles for free radical scavenging
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DOI：
10.2217/17435889.2.3.325
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发表时间：
2007-06-01
期刊：
影响因子：
5.5
通讯作者：
Sigmund, Wolfgang
中科院分区：
文献类型：
--
作者：
Tsai, Yi-Yang;Oca-Cossio, Jose;Sigmund, Wolfgang
目的：本文的目的是提出一种新的合成路线来形成 CeO2 纳米颗粒，以防止哺乳动物细胞氧化应激的有害影响。方法：使用非细胞毒性表面活性剂卵磷脂合成CeO2纳米粒子，并将产物在生物相容性柠檬酸三钠缓冲液中进行胶体稳定。这些纳米粒子被递送到小鼠胰岛素瘤β TC-tet 细胞中，并在各种细胞外 CeO2 浓度中测量对暴露于氢醌做出反应的细胞内自由基浓度。结果：获得了尺寸为 3.7 nm 的分散良好、高度结晶的 CeO2 纳米颗粒，其在 Dulbecco 改良 Eagle 介质中可以长时间保持化学和胶体稳定。用这些纳米粒子处理 β TC-tet 细胞可将有害的细胞内自由基水平降低至初级水平。结论：通过该途径合成的 CeO2 纳米粒子被证明是 β TC-tet 细胞内有效的自由基清除剂。此外，研究表明，CeO2 纳米颗粒提供了一种有效的方法来改善细胞在氧化应激导致的细胞损失限制天然功能的环境中的存活。
Aims: The aim of this article is to present a novel synthetic route to form CeO2 nanoparticles that protects against the detrimental influence of oxidative stress in mammalian cells. Methods: The noncytotoxic surfactant lecithin was used to synthesize CeO2 nanoparticles and the products were colloidally stabilized in a biocompatible tri-sodium citrate buffer. These nanoparticles were delivered into murine insulinoma beta TC-tet cells, and intracellular free radical concentrations responding to exposure to hydroquinone were measured in a variety of extracellular CeO2 concentrations. Results: Well-dispersed, highly crystallized CeO2 nanoparticles of 3.7 nm in size were achieved that are chemically and colloidally stable in Dulbecco's modified Eagle's medium for extended periods of time. Treating beta TC-tet cells with these nanoparticles alleviated detrimental intracellular free radical levels down to the primary level. Conclusion: CeO2 nanoparticles synthesized from this route are demonstrated to be effective free radical scavengers within beta TC-tet cells. Furthermore, it is shown that CeO2 nanoparticles provide an effective means to improve cellular survival in settings wherein cell loss due to oxidative stress limits native function.