Integrative omics approaches revealed a crosstalk among phytohormones during tuberous root development in cassava
Integrative omics approaches revealed a crosstalk among phytohormones during tuberous root development in cassava
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DOI：
10.1007/s11103-020-01033-8
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发表时间：
2020-08
影响因子：
5.1
通讯作者：
Yoshinori Utsumi;Maho Tanaka;Chikako Utsumi;S. Takahashi;A. Matsui;A. Fukushima;Makoto Kobayashi
中科院分区：
文献类型：
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作者：
Yoshinori Utsumi;Maho Tanaka;Chikako Utsumi;S. Takahashi;A. Matsui;A. Fukushima;Makoto Kobayashi
关键信息整合组学方法揭示了木薯块根发育过程中激素之间的相互作用。摘要木薯块根的形成是一个复杂的过程，包括阶段性变化以及细胞分裂和伸长的径向生长。我们进行了综合分析，以澄清在木薯块根形成过程中的代谢产物，植物激素和基因转录之间的关系。我们还证实了生长素（AUX）、细胞分裂素（CK）、脱落酸（阿坝）、茉莉酸（JA）、赤霉素（GA）、油菜素内酯（BR）、水杨酸和吲哚-3-乙酸与天冬氨酸结合物对块根发育的影响。对代谢产物和基因表达的综合分析表明，在块根发育过程中，参与淀粉生物合成和蔗糖代谢的几种编码酶的基因的表达水平上调，这与淀粉、糖磷酸盐和核苷酸的积累是一致的。植物激素和基因转录本的综合分析揭示了AUX信号，CK信号和BR信号之间的关系，与AUX，CK和BR诱导块根发育。阿坝和JA则抑制了块根的发育。这些现象可能代表茎块茎之间的差异（例如，马铃薯）和块根（例如，木薯）。在此基础上，建立了块根发育的激素调控模型。这个模型可能是有用的，为未来的植物激素研究涉及木薯。
Key MessageIntegrative omics approaches revealed a crosstalk among phytohormones during tuberous root development in cassava.AbstractTuberous root formation is a complex process consisting of phase changes as well as cell division and elongation for radial growth. We performed an integrated analysis to clarify the relationships among metabolites, phytohormones, and gene transcription during tuberous root formation in cassava (Manihot esculentaCrantz). We also confirmed the effects of the auxin (AUX), cytokinin (CK), abscisic acid (ABA), jasmonic acid (JA), gibberellin (GA), brassinosteroid (BR), salicylic acid, and indole-3-acetic acid conjugated with aspartic acid on tuberous root development. An integrated analysis of metabolites and gene expression indicated the expression levels of several genes encoding enzymes involved in starch biosynthesis and sucrose metabolism are up-regulated during tuberous root development, which is consistent with the accumulation of starch, sugar phosphates, and nucleotides. An integrated analysis of phytohormones and gene transcripts revealed a relationship among AUX signaling, CK signaling, and BR signaling, with AUX, CK, and BR inducing tuberous root development. In contrast, ABA and JA inhibited tuberous root development. These phenomena might represent the differences between stem tubers (e.g., potato) and root tubers (e.g., cassava). On the basis of these results, a phytohormonal regulatory model for tuberous root development was constructed. This model may be useful for future phytohormonal studies involving cassava.