Activity-induced changes in skeletal muscle metabolism measured with optical spectroscopy.
Activity-induced changes in skeletal muscle metabolism measured with optical spectroscopy.
复制标题
DOI：
10.1249/mss.0b013e31829a726a
复制
发表时间：
2013-12
影响因子：
4.1
通讯作者：
McCully KK
中科院分区：
文献类型：
--
作者：
Ryan TE;Southern WM;Brizendine JT;McCully KK
先前的研究使用近红外光谱（NIRS）来测量骨骼肌线粒体容量。这项研究检验了这样的假设：NIRS 测量的线粒体能力会随着耐力运动训练而提高，并随着停止训练而下降。九名年轻参与者进行了四个星期逐渐增加的腕屈肌耐力运动训练，然后大约五周不活动。每 3-7 天用 NIRS 测量一次肌肉耗氧量 (mVO2) 的恢复率，表明线粒体氧化能力。经过 4 周的运动训练后，线粒体容量（NIRS 速率常数）线性增加，组平均改善 64 ± 37%（p < 0.05）。运动训练停止后，线粒体能力呈指数下降，平均半衰期约为 7.7 天。用 NIRS 测量的线粒体对运动训练和停止训练的适应程度和时间进程与之前使用体外和体内技术的研究一致。这些发现表明，基于 NIRS 的测量可以检测线粒体容量的有意义的变化。
Previous studies have used near-infrared spectroscopy (NIRS) to measure skeletal muscle mitochondrial capacity. This study tested the hypothesis that NIRS measured mitochondrial capacity would improve with endurance exercise training and decline with detraining. Nine, young, participants performed four weeks of progressively increasing endurance exercise training of the wrist flexor muscles followed by approximately five weeks of inactivity. The rate of recovery of muscle oxygen consumption (mVO2) was measured with NIRS every 3-7 days, indicating mitochondrial oxidative capacity. A linear increase in mitochondrial capacity (NIRS rate constant) was found with a group average of 64 ± 37% improvement after four weeks of exercise training (p < 0.05). Mitochondrial capacity declined exponentially upon cessation of exercise training, with a mean half-time of ~7.7 days. Both the magnitude and time course of mitochondrial adaptations to exercise training and detraining measured with NIRS was consistent with previous studies using both in vitro and in vivo techniques. These findings show that NIRS based measurements can detect meaningful changes in mitochondrial capacity.